巨噬细胞来源的白细胞介素6对口腔鳞癌细胞增殖活性的影响

Macrophage-derived IL-6 promotes oral cancer cell proliferation

目的:探讨巨噬细胞来源的白细胞介素6(interleukin 6,IL-6)对口腔鳞状细胞癌细胞系增殖活性的影响。方法:通过MTT法检测不同浓度重组IL-6对口腔鳞状细胞癌细胞系SCC-15和CAL-27增殖活性的影响。建立佛波酯诱导的THP-1巨噬细胞分化模型,分别通过实时定量荧光PCR(RT-PCR)和酶联免疫吸附试验,分析脂多糖(lipopolysaccharide,LPS)刺激下的THP-1细胞中mRNA水平的变化以及THP-1细胞培养基中IL-6的含量。利用MTT法检测含THP-1条件培养基对SCC-15和CAL-27细胞系增殖活性的影响。采用GraphPad 7.0软件包对数据进行统计学分析。结果:培养基中添加IL-6,可促进SCC-15和CAL-27细胞系的增殖活性。LPS可刺激活化的THP-1巨噬细胞分泌IL-6;活化的THP-1细胞培养基可促进SCC-15和CAL-27细胞的增殖活性。结论:LPS刺激巨噬细胞分泌的IL-6,可促进口腔鳞状细胞癌细胞系SCC-15和CAL-27的增殖活性。

PURPOSE:To investigate the effect of macrophage-derived IL-6 on oral cancer cell proliferation.METHODS:The proliferation rates of SCC-15 and CAL-27 cells under different concentration of IL-6 were measured by MTT assay.THP-1 macrophages were induced by PMA,IL-6 mRNA level in LPS-stimulated THP-1 cells were measured by real-time qPCR,IL-6 protein contents in THP-1 conditional medium were measured by ELISA.SCC-15 and CAL-27 cells were co-cultured with THP-1 conditional medium,cell proliferation rate was measured by MTT assay.The data were analyzed by GraphPad 7.0 software package.RESULTS:Recombinant IL-6 in culture medium promoted SCC-15 and CAL-17 cell proliferation;LPS stimulated IL-6 expression in PMA-induced THP-1 macrophages.THP-1 conditional medium promoted SCC-15 and CAL-27 cell proliferation.CONCLUSIONS:IL-6 derived by LPS-stimulated macrophages could promote SCC-15 and CAL-27 cell proliferation.