植物人工启动子的研究进展

Research Progress of Artificial Promoter in Plant

人工启动子是调控基因表达的重要工具,通过改变拷贝数、顺式作用元件、转录因子结合位点(transcription factor binding sites,TFBS)间距及核心启动子序列等要素,可以人为地设计合成出易操作、理想化的驱动靶基因高水平表达的启动子。人工启动子可以定向调控转录活性,并驱动基因诱导表达、组织特异性表达和双向表达。本综述主要从合成人工启动子的方法和原理及植物基因工程中人工启动子的研究进展和应用前景进行介绍,以期为植物抗逆分子育种的研究提供参考。

As an important tool for regulating gene expression,artificial promoter is designed and synthesized to obtain easily operated and idealized promoter driving high-level expression of the target gene by changing elements such as the copy number,cis-acting element,TFBS spacing and core promoter sequence.Artificial promoters can target manipulation of transcriptional activity,and drive gene inducible expression,tissue-specific expression and bidirectional expression.In this review,the methods and principles of synthesis of artificial promoter,the research progress and application prospects of artificial promoters in plant genetic engineering were elaborated,in order to provide references for the research of plant stress resistance molecular breeding.