姜黄素诱导K562细胞自噬的抗瘤作用研究

Curcumin induced autophagy anticancer effects on chronic myelogenous leukemia cell line K562

目的研究姜黄素诱导自噬对K562细胞增殖的作用。方法采用MTT法检测不同浓度的姜黄素及自噬特异性抑制剂3-MA对K562细胞存活率的影响;通过Western blot法检测自噬标志蛋白Beclin1及LC3-Ⅱ/LC3-Ⅰ的水平评价自噬;应用实时定量PCR法检测BCR-ABL融合基因的表达。结果姜黄素以剂量、时间依赖方式抑制K562细胞增殖;与加入3-MA的对照组相比,Beclin1的水平及LC3-Ⅱ/LC3-Ⅰ的比值明显升高;姜黄素可导致BCRABL融合基因的水平降低。结论姜黄素诱导自噬可抑制K562细胞增殖并下调BCR-ABL融合基因的表达水平。

Objective To investigate the proliferative effect of curcumin-induced autophagy on chronic myelogenous leukemia cell line K562.Methods K562cells was incubated with various concentrations of curcumin and autophagyspecific inhibitor 3-MA,the cell viability was measured by MTT;the level of autophagy marker protein Beclin1and the ratio of LC3-Ⅱ/LC3-Ⅰwere detected by Western blot to evaluate autophagy;mRNA expression level of BCR-ABL fusion gene was determined by quantitative real-time PCR.Results Dose-and time-dependent K562cell viability inhibition was observed following curcumin treatment;the level of Beclin1and the ratio of LC3-Ⅱ/LC3-Ⅰwere significantly increased compared with the control group with 3-MA;in addition,curcumin reduced the level of BCR-ABL fusion gene.Conclusion Curcumin could induce autophagy to inhibit the proliferation of K562cells and downregulate expression level of BCR-ABL fusion gene.