巨噬细胞:一种理想的细胞骨架观察教学材料

Macrophage: An Ideal Teaching Material for Cytoskeleton Observation

细胞骨架是细胞的重要结构之一,也是《细胞生物学实验》教学的重要内容。然而,在目前的教学实践中,缺乏较为理想的细胞骨架观察材料。基于对巨噬细胞易黏附、便于操作特性的了解,该研究以小鼠腹腔巨噬细胞为材料,进行了细胞骨架显示的实验探索。结果表明,无论用曲通X-100处理细胞后再用考马斯亮蓝染色还是先对细胞进行固定、透膜后再用免疫荧光法染色,均可观察到清晰的细胞骨架结构。说明巨噬细胞是一种理想的细胞骨架观察教学材料。

Cytoskeleton is one of the important structure of cells,also is the important content of the course of Cell Biology Experiment.However,there is a lack of ideal cell materials for cytoskeleton observation in the current teaching practice.According to our understanding of the characteristics of easy adhesion and operation of macrophages,we used mouse abdominal macrophages as materials to observe cytoskeleton for experimental exploration.The results showed that clear cytoskeleton structure could be observed whether the cells were treated with Triton X-100 and then stained with coomassie brilliant blue or the cells were fixed,permeabilized,and stained with immunofluorescence.It shows that macrophage is an ideal teaching material for cytoskeleton observation.