肺癌患者化疗前后血浆let-7a表达水平与化疗药物疗效相关性分析

Analysis of the correlation between the expression of let-7a in plasma and the therapeutic effect of chemotherapy in lung cancer patients before and after chemotherapy

目的:探讨肺癌患者化疗前后血浆中let-7a表达水平,分析其与化疗药物疗效的相关性。方法:收治肺癌晚期患者60例,采用实时荧光定量聚合酶链反应法(qRT-PCR)检测let-7a水平。比较化疗前后let-7a水平变化,观察血浆let-7a表达水平与顺铂联合5-Fu/CF化疗方案疗效的相关性。结果:化疗后血浆let-7a水平较化疗前降低,差异有统计学意义(P<0.05)。化疗前血浆let-7a在CR+PR的表达量明显低于PD+SD,差异有统计学意义(P<0.05)。结论:肺癌晚期患者let-7a表达水平与化疗药物疗效具有相关性。血浆let-7a有可能成为预测化疗药物疗效的潜在性生物指标。

Objective:To investigate the expression of let-7a in the plasma of lung cancer patients before and after chemotherapy,and to analyze the correlation between it and the therapeutic effect of chemotherapy drugs.Methods:We used real-time fluorescence quantitative polymerase chain reaction(QRT-PCR)to detect 60 patients with advanced lung cancer.We compared the level of let-7a before and after chemotherapy,and observed the correlation between the level of plasma let-7a expression and the efficacy of cisplatin combined with 5-FU/CF chemotherapy.Results:After chemotherapy,the level of let-7a in the plasma was lower than that before chemotherapy,the difference was statistically significant(P<0.05).Before chemotherapy,the expression of let-7a in the(Cr+PR)group was significantly lower than that in the(PD+SD)group,the difference was statistically significant(P<0.05).Conclusion:The expression level of let-7a in patients with advanced lung cancer was correlated with the therapeutic effect of chemotherapy.Plasma let-7a may be a potential biomarker to predict the efficacy of chemotherapy drugs.