几种环境因子对崖州硬皮豆种子萌发的影响

Effects of several environmental factors on seed germination of Macrotyloma uniflorum ‘Yazhou’

本研究运用培养皿滤纸法和盆栽法,分析了光照、温度、水分、盐分和埋藏深度等环境因子对崖州硬皮豆(Macrotyloma uniflorum‘Yazhou’)种子萌发的影响。结果表明,光照、温度及其互作显著影响种子萌发率(P<0.05),随着温度升高,种子萌发率和萌发速率(1/t50)先上升后下降,光照和黑暗条件下的最适萌发温度分别为32.8和32.4℃;种子萌发率和萌发速率(1/t50)随水势胁迫和NaCl胁迫的增加呈下降趋势,其中萌发速率(1/t50)较萌发率下降快;当渗透势和盐分浓度分别为-1.2 MPa和400 mmol·L^-1时,种子几乎不萌发;崖州硬皮豆种子在埋藏深度1 cm时出苗率最高,适宜浅播。

The effects of light,temperature,water,salinity,and burial depth on seed germination of Macrotyloma uniflorum‘Yazhou’was analyzed using petri-dish and pot-culture methods in this study.The results indicated that light,temperature,and their interaction significantly affected seed germination percentage(P<0.05).Germination percentage and germination rate(1/t50)first increased and then declined with increasing temperature.The seeds of M.uniflorum‘Yazhou’could germinate between 15~40℃,but the optimum germination temperature was 32.8℃under light conditions and 32.4℃under dark conditions.With increasing PEG and NaCl stress,the germination percentage and germination rate(1/t50)gradually decreased,although germination rate(1/t50)decreased faster than did germination percentage.The seeds of M.uniflorum‘Yazhou’could not germinate when the osmotic potential and NaCl concentration reached-1.2 MPa and 400 mmol·L^-1,respectively.The seedling percentage was highest at 1 cm burial depth,indicating that M.uniflorum‘Yazhou’is possibly suitable for shallow seeding.