响应面法优化提纯牛肉肌红蛋白

Extracted and Purified Myoglobin in Beef Using Response Surface Method

以牛背肌肉为原料,筛选Tris-HCl复合提取液pH、提取液浓度、提取时间、料液比、硫酸铵溶液饱和度等对肌红蛋白提取有影响的因素,并从中选出具有显著影响的因素(提取液pH、提取液浓度、硫酸铵溶液饱和度)进行响应面优化试验,确定牛肉肌红蛋白提取最优工艺参数提取液pH为8.15、硫酸铵溶液饱和度90.93%、提取时间1.63 h。对粗提蛋白进行纯化及层析交换分离,得到纯度较高的肌红蛋白含量2.14 mg/mL,总蛋白浓度2.39 mg/mL,样品纯度达到91.95%。

In this study,Longissimus dorsi of cattle was used as the material,and the factors of affect the extraction of myoglobin were screened,such as pH value of Tris-HCl composite extracting solution,extract concentration,extracting time,solid-liquid ratio,ammonium sulfate saturation. The factors with significant influence(pH of the extract,concentration of the extract,saturation of ammonium sulfate solution)were selected for response surface optimization test,and the optimal extraction parameters of beef myoglobin were determined:pH value of extracting solution was 8.15,saturation of ammonium sulfate solution was 90.93 %,and extraction time was 1.63 h. The crude protein was dialysis purification and separation by chromatography exchange,to obtain the high purity myoglobin content was 2.14 mg/mL,the total protein concentration was 2.39 mg/mL,and the purity of the sample reached 91.95%.