违法犯罪人员DNA建库中检测板的四种复核方法

Four Methods to Check the Position of 96-well Plate for Sampling in Constructing DNA Database of Criminals

违法犯罪人员DNA数据库建设工作内容繁杂、工作量大,故必须避免实验操作过程中的人工失误,为此需有一套复核和自纠程序。因为在检测环节,不排除可能会发生错摆各板的次序而导致检测样本和已导入基因分析仪运行软件中的跑样表均无法“对号入座”,最终使各板都结果错误的情况。如此,既影响工作质量和效率,又会浪费试剂和耗材。对此,结合日常建库工作积累的经验,本文总结了四种复核方法:前后板间连锁复核法、计算机C语言板间复核程序法、本板内选定孔号复核法和“假入库”复核法。四种方法各有所长,甚至可联合应用,从而提高板间复核的准确率,保证违法犯罪人员DNA数据库的准确性和可靠性。

The construction of DNA database of criminals is complicated and large of workload,likely rendering the occurrence of manual errors during experimental process,therefore a set of review and self-correction procedures must be built up in order to rule out the possibility of erroneously placing the sampling 96-well plate.Thus,the tested samples are able to“sit in right seat”against the running table that has been introduced into a genetic analyzing software,avoiding the errors emerging so as to secure the work quality and effi ciency and prevent the waste of man efforts and consumables.Based on the accumulated experiences from our daily database operation,four review methods are here summarized:1.reviewing between 96-well plates with the selected samples in previous added plate through testing the designated wells of the immediate following plate;2.programming by computer C language to carry out the task of 1;3.selecting samples to be added and tested in the other wells of same one plate;4.checking the consistency of the tested individual’s data against his/her already-stored background information in the database.The four methods have their respective advantages,capable of their combinatorial application to improve the accuracy of inter-plate review and ensure the reliability of criminal DNA database.