摘要
Objective To explore the pharmacodynamic mechanism of Fu Zi Li Zhong Decoction(FZLZD)in treating gastric ulcer and its effect on gastrointestinal microecology.Methods Rat gastric ulcer model was established by continuous vinegar gavage.The experiment included normal group,model group,ranitidine group and FZLZD group.The animals were handled after intragastricing administration of corresponding clinical isodose drugs for 7 d.The rats’gastrointestinal histopathology was observed by HE staining.The expression of ZO-1 and Occludin mRNA in rat gastric tissue was detected by RT-PCR.The diversity of intestinal flora was analyzed by 16SrDNA sequencing.Results FZLZD can effectively improve gastric mucosal destruction,epithelial exfoliation,inflammatory cell infiltration,inflammatory substance exudation and edema in rats with gastric ulcer.FZLZD can effectively up-regulate the expression levels of ZO-1 and Occludin mRNA in gastric tissue of rats with gastric ulcer.The results of intestinal flora diversity showed that the diversity of intestinal flora in FZLZD group was significantly higher than that of model group.Conclusions FZLZD can regulate gastric ulcer by promoting the expression of ZO-1 and Occludin and improving the diversity of intestinal flora.
目的探讨附子理中汤治疗胃溃疡的药效机制和对胃肠道微生态的影响。方法用食醋连续灌胃建立大鼠胃溃疡模型,实验设空白组、模型组、雷尼替丁组、附子理中汤组。灌胃给予相应的临床等剂量药物7 d后,处理实验动物。用HE染色观察大鼠胃肠组织病理;RT-PCR法检测大鼠胃组织中ZO-1、Occludin mRNA表达情况;通过16SrDNA测序法分析肠道菌群多样性变化。结果附子理中汤能有效改善胃溃疡大鼠的胃黏膜破坏、上皮脱落,炎细胞浸润,炎性物质渗出及水肿等情况;附子理中汤能有效上调胃溃疡大鼠胃组织中ZO-1、Occludin mRNA的表达水平;肠道菌群多样性结果显示,与模型组相比,附子理中汤组中肠道菌群多样性明显升高。结论附子理中汤能通过促进ZO-1、Occludin的表达和改善肠道菌群多样性来调理胃溃疡。
基金
funding support from the National Natural Science Foundation of China (No. 81973670 and No. 81503445)
Hunan Provincial Natural Science Foundation of China (No. 2016JJ2095)
Hunan Province College Students Research Learning and Innovative Experiment Project (No. 2018-412)
