摘要
目的分析不同浓度CAL-101对Burkitt淋巴瘤(BL)细胞系Raji和弥漫大B细胞淋巴瘤(DLBCL)细胞系SUDHL-10增殖的抑制作用及其相关机制。方法通过不同浓度(5μmol/L、10μmol/L、15μmol/L、20μmol/L)的CAL-101对BL细胞系Raji和DLBCL细胞系SUDHL-10进行处理,采用MTT法测定CAL-101对上述淋巴瘤细胞系增殖的抑制作用,用流式细胞术对淋巴瘤细胞凋亡情况进行测定,通过迁移实验测定CAL-101淋巴瘤细胞迁移情况的影响,并通过Western blot法对CAL-101处理后淋巴瘤细胞表达磷酸化ERK的变化进行检测。结果在CAL-101浓度≥5μmol/L时,对DLBCL细胞系SUDHL-10和BL细胞系Raji增殖具有良好的抑制作用,随着CAL-101浓度的升高,两种淋巴瘤细胞系的增殖抑制率亦明显升高(P<0.01),具有浓度依赖性的特点。随着CAL-101浓度的升高,两种淋巴瘤细胞系的细胞凋亡率亦明显升高(P<0.01),具有浓度依赖性的特点。随着CAL-101浓度的升高,CAL-101对两种淋巴瘤细胞系的细胞迁移率亦明显降低(P<0.01),具有浓度依赖性的特点。Western blot法分析结果显示,经CAL-101处理淋巴瘤细胞后,可明显降低磷酸化ERK的表达水平。结论CAL-101能够明显阻滞BL细胞系Raji和DLBCL细胞系SUDHL-10增殖,促使细胞凋亡,阻滞细胞迁移至淋巴瘤基底细胞,其发生机制可能与ERK信号途径阻断密切相关。
Objective To analyze the inhibitory effect and related mechanism of different concentrations of CAL-101 on proliferation of Burkitt lymphoma(BL)cell line Raji and diffuse large B cell lymphoma(DLBCL)cell line SUDHL-10.Methods Raji of BL cell line and SUDHL-10 of DLBCL cell line were treated by different concentration of CAL-101,inhibition effect of CAL-101 on the proliferation of the lymphoma cell lines was determined by MTT method;the apoptosis of lymphoma cells was determined by flow cytometry;the effect of CAL-101 on the migration of lymphoma cells was detected by migration experiment and the expression of phosphorylated ERK on lymphoma cells treated with CAL-101 was detected by Western blot method.Results The CAL-101 showed significant inhibitory effect on the proliferation of SUDHL-10 in DLBCL cell line and Raji in BL cell line when the concentration was≥5 mol/L;with the increase of CAL-101 concentration,the inhibition rate of the two kinds of lymphoma cell lines proliferation increased significantly(P<0.01)in a concentration dependent manner.Also with the increase of CAL-101 concentration,the apoptosis rate of the two kinds of lymphoma cell lines increased significantly(P<0.01)in a concentration dependent manner.With the increase of CAL-101 concentration,the cell migration rate of the two kinds of lymphoma cell lines also decreased significantly(P<0.01)in a concentration dependent manner.The results of Western blot analysis showed that the expression of phosphorylated ERK on lymphoma cells treated with CAL-101 was significantly reduced.Conclusion CAL-101 can significantly block the proliferation of Raji in BL cell line and SUDHL-10 in DLBCL cell line,promote cell apoptosis,hinder cell migration to lymphoma basal cells,and the mechanism may be closely related to the block of ERK signaling pathway.
作者
封晓荣
王娜
叶莎
张澍
李冰
张岁龙
FENG Xiao-rong;WANG Na;YE Sha(Department of Geriatric Cardiology,The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an Shaanxi 710004,China;Department of Research Center Laboratory,The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an Shaanxi 710004,China)
出处
《临床和实验医学杂志》
2019年第23期2510-2514,共5页
Journal of Clinical and Experimental Medicine
基金
陕西省社发公关项目(编号:2010K15-01-08)