MAPREC与MNVT评价Ⅲ型Sabin株脊髓灰质炎病毒神经毒力的比较

Evaluation of the neurovirulence of type Ⅲ Sabin strain poliovirus through MAPREC and MNVT

目的用PCR扩增及限制性酶切分析突变技术(MAPREC)和猴体神经毒力试验(MNVT)评价同一批Ⅲ型Sabin株脊灰病毒神经毒力的结果差异。方法采用MAPREC技术,检测Ⅲ型Sabin株脊灰病毒神经毒力关键位点472位点突变率;采用MNVT病理切片分析,评价Ⅲ型Sabin株脊灰病毒神经毒力。结果 MAPREC检测待测样品核酸472位点突变率均值为0.324%,低于高突变参考品的0.621%。MNVT切片结果分析表明,xtest–xref=0.169,小于C1。对同一批样品,MAPREC与MNVT分析结果一致。结论在Ⅲ型Sabin株脊灰病毒神经毒力检测方面,操作简单,试验周期短的MAPREC技术可作为金标准MNVT的有益补充。

Objective To study the difference between the neurovirulence of the same type Ⅲ Sabin strain poliovirus examined by MAPREC and MNVT. Methods The MARPEC assay was used to detect the point mutation rate of the 472 site, which are the key sites of the virulence of the type Ⅲ Sabin strain poliovirus. The paraffin sections of the MNVT was analyzed to evaluate the neurovirulence of the type Ⅲ Sabin strain poliovirus. Results The average mutation rate of 472 site in MAPREC assay was 0.324%, which was lower than 0.621% of HMVR. The paraffin sections results of MNVT showed that xtest xref = 0.169, and the result was less than C1. The results of MAPREC of the same batch of samples were consistent with the results of MNVT. Conclusion In the detection of virulence of poliovirus type Ⅲ Sabin strain, the simple operation and short test period of MAPREC can be used as a beneficial supplement to the gold standard MNVT.