TNF-α和IL-6在高原地区翼状胬肉组织中的表达研究

Expression of TNF-α and IL-6 in pterygium tissues of plateau area

目的探讨高原地区与平原地区翼状胬肉组织中TNF-α和IL-6的表达情况。方法免疫组织化学法和Western blot法检测29例平原地区和29例高原地区翼状胬肉患者切除的翼状胬肉组织中的TNF-α及IL-6的表达。结果免疫组织化学法结果显示,TNF-α位于结膜上皮细胞基底膜和胞浆,IL-6位于结膜上皮细胞、增生的纤维组织内及血管旁炎症细胞胞浆中。TNF-α在高原组的阳性表达率(86.21%)显著高于在平原组中的阳性表达率(58.62%),差异具有统计学意义(P<0.05);IL-6在高原组的阳性表达率(82.76%)显著高于在平原组中的阳性表达率(55.12%),差异具有统计学意义(P<0.05)。Western blot检测显示TNF-α及IL-6在高原及平原翼状胬肉组织均有表达,在高原翼状胬肉组织中表达增强。结论TNF-α和IL-6在高原地区翼状胬肉组织中的表达增强,可能与高原特殊环境有关。

Objective To investigate the expression of TNF-αand IL-6 in pterygium of plateau area and plain area.Methods Immunohistochemistry and Western blot were used to detect the expression of TNF-αand IL-6 in the excised pterygium tissues of 29 patients with pterygium in plain area and 29 patients with pterygium in plateau area.Results Immunohistochemical results showed that TNF-αwas located in the basement membrane and cytoplasm of conjunctival epithelial cells,while IL-6 was located in the cytoplasm of conjunctival epithelial cells,proliferated fibrous tissues and perivascular inflammatory cells.The positive expression rate of TNF-αin plateau group(86.21%)was significantly higher than that in plain group(58.62%).The difference was statistically significant(P<0.05).The positive expression rate of IL-6 in plateau group(82.76%)was significantly higher than that in plain group(55.12%).The difference was statistically significant(P<0.05).The results from Western blot analysis showed that TNF-αand IL-6 were expressed in plateau and plain pterygium tissues,but increased in plateau pterygium tissues.Conclusions The expression of TNF-αand IL-6 is increased in pterygium tissues of plateau area,which may be related to the special environment of the plateau.