抗Ⅱ型胶原抗体水平与血清阴性类风湿关节炎疾病活动相关性分析

Anti-type Ⅱ collagen antibody level and its implications in seronegative rheumatoid arthritis

目的:探讨抗Ⅱ型胶原(CⅡ)抗体在血清阴性类风湿关节炎(SNRA)中的可行性。方法:用ELISA法检测35例SNRA患者以及15例非RA的其他风湿病患者和10例健康人群的血清抗CⅡ抗体水平。结果:①SNRA患者抗CⅡ抗体阳性率为28.57%,其他风湿病患者中为6.67%,健康人为0,差异有统计学意义(P<0.05);②抗CⅡ抗体阳性SNRA患者C反应蛋白(CRP)和血沉(ESR)、DAS28评分较抗CⅡ抗体阴性患者升高(P<0.05);③35例SNRA患者平均C-Ⅱ水平与ESR呈正相关(r=0.394,P=0.019),与CRP(r=0.1,P=0.566)、DAS28评分(r=0.287,P=0.094)之间无显著相关性;④不同X射线分期患者血清抗CⅡ抗体水平差异有统计学意义(P<0.05)。结论:抗CⅡ抗体与SNRA的疾病活动度有相关性,且抗CⅡ型胶原抗体的检测有助于提高SNRA的诊断,可作为辅助诊断和监测疗效的指标。

Objective:To investigate the clinical implications of anti-typeⅡcollagen antibody level in seronegative rheumatoid arthritis(SNRA).Methods:Serum anti-CⅡantibody levels were measured in 35 SNRA patients and 15 with other rheumatic diseases as well as 10 healthy control subjects using enzyme linked immunosorbent assay(ELISA).Results:①Positive rate of anti-CⅡantibody was 28.57%in SNRA patients,6.67%in other rheumatism and 0%in healthy controls.The difference was significant(P<0.05);②SNRA patients with positive anti-collagenⅡantibody had higher C-reactive protein(CRP)level,erythrocyte sedimentation rate(ESR)and scoring by rheumatoid arthritis DAS28 than those with negative anti-CⅡantibody(P<0.05);③The average anti-CⅡantibody level in 35 SNRA patients was positively correlated with ESR(r=0.394,P=0.019),yet had no significant correlation with CRP(r=0.1,P=0.566)and DAS28 scoring(r=0.287,P=0.094);④There was significant difference in serum anti-CⅡantibody levels among patients with different X-ray stages(P<0.05).Conclusion:Anti-CⅡantibody level is associated with disease activity of SNRA,and can be used as an auxiliary index to improve diagnosis and evaluate the disease progress.