人RPS6KA3基因及蛋白质的生物信息学分析

Bioinformatic analysis of human RPS6KA3 gene and protein

核糖体蛋白S6激酶A3(ribosomal protein S6 kinase A3,RPS6KA3)具有丝氨酸/苏氨酸激酶活性,在机体的生理过程中发挥重要作用。为了研究RPS6KA3基因的性质和功能,利用一系列生物信息学分析软件对该基因序列及其编码蛋白的结构和特性进行生物信息学分析。结果表明,人RPS6KA3基因共编码740个氨基酸组成的多肽,其在进化过程中高度保守,隶属于PKc_like超家族,是一种等电点为6.41的不稳定的水溶性蛋白,无信号肽序列和跨膜结构。该蛋白定位于细胞质的可能性最大,主要的二级结构为α-螺旋结构,具有多个磷酸化功能位点。与RPS6KA3相互作用的蛋白主要是MAPK信号途径相关蛋白、mTOR信号途径相关蛋白及蛋白合成相关蛋白等。分析结果为进一步研究RPS6KA3在生命过程中的作用提供重要信息。

Ribosomal protein S6 kinase A3(RPS6KA3)has Serine/Threonine kinase activity that plays an important role in the body′s physiological processes.In order to study the nature and function of gene RPS6KA3,bioinformatics analysis of its gene sequence and the structure and properties of its encoded protein was analyzed by a series of bioinformatics analysis software in this paper.The results showed that the human RPS6KA3 gene encoded a polypeptide comprised of 740 amino acids residues,which was highly conserved in the evolutionary process and belonged to the PKc_like super family.It was an unstable and water-soluble protein with isoelectric point of 6.41 without signal peptide sequence and trans-membrane region detected in this protein.It was mainly located in the cytoplasm,and the main secondary structure elements were alpha helix structure with several phosphorylation sites.Interactive proteins with RPS6KA3 were mainly proteins associated with MAPK and mTOR signaling pathway and protein synthesis.Our results could provide important messages for further studing on the function of RPS6KA3 in the life process.