6个玉簪品种的抗氧化酶特性及同工酶分析

Analysis on antioxidant enzyme activities and peroxidase isozymes in six Hosta varieties

以6个玉簪品种为试验材料,研究了不同玉簪品种幼苗抗氧化酶活性及其同工酶谱的差异性。结果表明:在非胁迫环境中,品种‘火与冰’和‘梦想’的SOD,POD活性显著高于其他品种;CAT活性品种间差异不显著。同工酶谱分析表明:6个玉簪品种叶片SOD和CAT同工酶谱带相似;不同品种在POD同工酶带型分布及显色强度上存在差异;品种‘火与冰’和‘梦想’均存在其特征谱带。总体认为,品种‘火与冰’和‘梦想’抗逆性较强。

Antioxidant enzyme activities of 6 Hosta varieties including H.‘Fire and ice’,H.‘Dream’,H.‘Frances Williams’,H.‘Fran Godfrey’,H.‘Aureomarginata’and H.‘Patriot’were measured,and the isozymes of 6 samples were studied by polyacylamide gel electrophoresis.The results showed that there was no obvious difference in the activity of CAT in the leaves of 6 Hosta varieties.Moreover,the highest activities of SOD and POD were found in H.‘Fire and ice’and H.‘Dream’.The data indicated that isozyme profiles of SOD and CAT were similar in 6 varieties.There were differences in the band pattern and color intensity of the isozyme profiles of POD in different varieties,and H.‘Fire and ice’and H.‘Dream’had those own characteristic bands.We concluded that the resistances of H.‘Fire and ice’and H.‘Dream’were stronger.