血液灌流吸附材料组织相容性和有效性的体外实验

In vitro study of histocompatibility and clearance of hemoperfusion adsorbents

目的通过体外实验方法探索血液灌流中常用的吸附材料(活性炭和吸附树脂)对血常规和生化指标的影响和对血中毒死蜱及其代谢产物的清除效果。方法采集志愿者外周静脉血分别与活性炭和吸附树脂吸附剂混合孵育,检测其中的血常规和生化指标的变化情况,评价吸附材料的组织相容性。采集志愿者外周静脉血加入毒死蜱及其代谢产物后分别与活性炭和吸附树脂吸附剂混合孵育,检测其中毒死蜱及其代谢产物的变化情况,评价吸附材料的有效性。结果活性炭和吸附树脂吸附剂降低血小板、葡萄糖、白蛋白、尿酸、肌酐、三碘甲腺原氨酸、游离三碘甲腺原氨酸的含量(F=3.671、10.564、5.239、7.175、23.673、11.161、10.256,均P<0.05),对其他血常规和生化指标无明显影响。活性炭和吸附树脂吸附剂均可以降低血中毒死蜱及其代谢产物的浓度(F=798.110、1495.212,P<0.01)。结论在体外实验条件下活性炭和吸附树脂对大多数血常规、生化指标无明显影响,均具有良好的组织相容性,活性炭和吸附树脂均可有效清除毒死蜱及其代谢产物。

Objective To evaluate the histocompatibility and clearance of chlorpyrifos and its metabolite of activated charcoal and adsorption resin by in vitro study.Methods Venous blood from volunteers were incubation with activated charcoal or adsorbent resins,cytometry parameters and plasma components were detected for evaluation the histocompatibility of adsorbents.Venous blood from volunteers mixed with chlorpyrifos and its metabolite were incubation with activated charcoal or adsorbent resins,plasma concentration of chlorpyrifos and its metabolite were detected for evaluation the efficacy of adsorbents.Results Incubation tests show that the absorbents reduce the blood platelet(F=3.671,P<0.05),serum glucose(F=10.564,P<0.05),albumin(F=5.239,P<0.05),uric acid(F=7.175,P<0.05),creatinine(F=23.673,P<0.05),T3(F=11.161,P<0.05)and free T3(F=10.256,P<0.05).However,other cytometry parameters and plasma components were not influenced.Both activated charcoal and adsorbent resins could reduce the plasma concentration of chlorpyrifos(F=798.110,P<0.01)and its metabolite(F=1495.212,P<0.05).Conclusion In vitro test show that both activated charcoal and adsorbent resins could clear chlorpyrifos and its metabolite,however,could not influence main cytometry parameters and plasma components,the histocompatibility of adsorbents are satisfactory.