基于液质联用技术采用正交设计优选制川乌单酯型生物碱醇提工艺

Optimization of Ethanol Extraction Technology of Monoester Alkaloids from Aconiti Radix Cocta Based on HPLC-MS and Orthogonal Test

目的优选制川乌单酯型生物碱的醇提工艺。方法采用液质联用技术结合正交试验,以单酯型生物碱(以苯甲酰新乌头原碱、苯甲酰次乌头原碱、苯甲酰乌头原碱3种特征成分之和计)含量为指标,在确定提取次数的基础上,对乙醇浓度、乙醇用量、提取时间3个因素进行考察,优选单酯型生物碱提取工艺。结果最佳醇提工艺为乙醇浓度75%,乙醇25倍用量,提取2次,每次1.5 h。结论优化的提取工艺简单可行、稳定可靠,对制川乌单酯型生物碱的工业化大生产与质量控制具有一定的参考意义。

Objective To optimize the ethanol extraction technology of monoester alkaloids from Aconiti radix cocta.Methods On the basis of defined extraction times,ethanol concentration,ethanol dosage and extraction time were investigated by HPLC-MS combined with orthogonal test to optimize extraction process using content of monoester alkaloids(the sum of benzoyl neoaconitine,benzoyl hypoaconitine and benzoyl aconitine)as index.Results The optimum ethanol extraction technology was as follows:ethanol concentration was 75%,and ethanol dosage was 25 times of medicinal material.The medicinal material was extracted for 2 times,1.5 h for each time.Conclusion The optimal extraction technology is simple,feasible,stable and reliable.It can provide reference for the industrial production and quality control of monoester alkaloids from Aconiti radix cocta.