番茄叶斑病和果斑病病原鉴定及生物学特性研究

Pathogen identification and biological characteristics of pathogens of tomato leaf spot and fruit spot disease

从武汉市番茄大棚番茄叶片和果实上分离出引起番茄叶斑病和果斑病的病原真菌,对该菌进行了病原菌鉴定和生物学特性研究。结果表明,经形态学观察、致病性测定及分子鉴定该病原菌为多主棒孢霉(Corynespora cassiicola)。对该菌株生物学特性研究表明,该病原菌喜光照,最适宜生长的温度为25~30℃,生长适宜pH为7~9,喜欢弱碱性生长环境,供试5种碳源中葡萄糖的利用效果最好,5种氮源中硝酸钾利用效果最好。

Pathogenic fungi causing tomato leaf spot and fruit spot disease were isolated from tomato leaves and fruits in greenhouse of Wuhan city. The pathogen identification and biological characteristics were studied. The results showed that the pathogen of tomato leaf spot and fruit spot disease was identified as Corynespora cassiicola by morphological observation,pathogenicity determination and molecular identification. The biological characteristics of the strain showed that the pathogen liked light, the optimum temperature for growth was 25~30 ℃, the optimum pH was 7~9, and the weak alkaline growth environment was preferred. Among the five carbon sources, glucose had the best utilization effect, and potassium nitrate had the best utilization effect among the five nitrogen sources.