一株鸡源新城疫病毒的分离鉴定

Isolation and Identification of A Newcastle Disease Virus from Chicken

从湖北某养殖厂一只死亡鸡只中分离出一株病毒,通过血凝实验、血凝抑制实验和PCR方法对其进行了分离鉴定。结果表明,该病毒能与1%鸡红细胞发生凝集反应,并且该病毒液能被新城疫标准阳性血清所抑制,而不能被禽流感标准阳性血清所抑制,新城疫病毒的F基因PCR引物扩增出特异性条带,证明分离株为新城疫病毒,命名为HB1910。分离毒的血凝价为1:512,EID50为107.83∕0.1 mL用琼脂糖凝胶电泳对PCR产物进行检测,结果分离毒株在约350 bp处均可见到清晰的目的条带,而禽流感引物扩增无条带,证明分离毒为鸡新城疫病毒。

A virus was isolated from a dead chicken in a breeding factory in Hubei Province.It was isolated and identified by hemagglutination test,hemagglutination inhibition test and PCR.Through the test,the virus can aggluti nate with 1%chicken red blood cells,and the virus solution can be inhibited by the standard positive serum of newcas tle disease,but not by the standard positive serum of avian influenza The specific bands were amplified by PCR prim ers of F gene of the virus,which proved that the isolate was Newcastle disease virus,named HB1910.The hemaggluti nation value of the isolate was 1:512 and EID50 was 107.83/0.1mL.The PCR products were detected by agarose gel elec trophoresis.Results showed that the target bands were visible at about 350 bp,but no bands were found in the primers.