壮药白花九里明干预心肌损伤和保护心肌细胞作用的研究

Zhuang Medicine Blumea Megacephala on Research by Intervention of Myocardial Injury and Protects of Heart Cells

目的:探讨白花九里明在心肌缺血损伤的干预保护作用及作用机制。方法:60只小鼠分为:正常对照组和模型对照组(给予0.9%氯化钠注射液灌胃),硝酸甘油组(给予13 mg/mL灌胃),白花九里明提取液低、中、高浓度组(分别给予150、300、600 mg/mL灌胃);同时正常对照组给予0.9%氯化钠注射液,其余各组给予垂体后叶素注射液0.26 U/mL腹腔注射;连续用药15 d,检测小鼠心电图、心肌总蛋白(TP)、谷草转氨酶(AST)、过氧化物歧化酶(SOD)、丙二醛(MDA)及心肌组织形态。结果:模型对照组的P波时限明显小于其余各组(P<0.01)、J点上移明显高于其余各组(P<0.01)。正常对照组的心率明显低于其余各组(P<0.01,0.05)。白花九里明中浓度组的TP浓度明显高于正常对照组、硝酸甘油组、白花九里明低浓度组,SOD活性明显低于这些组(P<0.01,0.05);白花九里明高浓度组的TP浓度明显高于其余组、SOD活性明显低于其余组(P<0.01);白花九里明中浓度组与高浓度组的AST浓度无统计学差异,而均明显低于其余各组(P<0.01)。白花九里明高浓度组和正常对照组的MDA比较无统计学差异,而又明显低于其余组(P<0.01,0.05);白花九里明中浓度组的MDA明显低于白花九里明低浓度组(P<0.05)。模型对照组心肌细胞数量明显减少,心肌细胞间毛细血管明显扩张,细胞变性坏死区域明显;硝酸甘油组、白花九里明低浓度组和中浓度组的心肌细胞结构优与模型对照组,心肌细胞间毛细血管扩张和变性坏死区域等均有较大的改善;白花九里明高浓度组的心肌细胞结构清晰,没有明显变性坏死和毛细血管扩张现象,与正常对照组心肌细胞结构表达相接近并明显优于白花九里明低浓度组和中浓度组。结论:白花九里明有改善心肌细胞缺血缺氧、干预氧自由基对心肌细胞损伤、促进心肌细胞修复作用,这些作用存在量效正相关。

Objective Explore the protective effect intervention and mechanism of Blumea megacephala on myocardial injury in mice.Methods Sixty mice divided:Normal control group and Model control group(give 0.9%NaCl injection),Nitroglycerine group(give 13 mg/mL Nitroglycerine),Blumea megacephala low,medium and high concentration groups(respectively give 150,300,600 mg/ml Blumea megacephala extract);Intraperitoneal injection:Normal control group give 0.9%NaCl injection,Other groups give 0.26 U/mL pituitrin injection.Continuous use of 15 d,detection ECG of mice,detection TP、AST、SOD、MDA and tissue section in the heart.Results Model control group the P wave duration obviously shorter than other groups(P<0.01),the J point move up obviously higher than other groups(P<0.01).Normal control group the heart rate obviously below than other groups(P<0.01,0.05).Blumea megacephala medium concentration group the TP obviously higher than Normal control group,Nitroglycerine group,Blumea megacephala low concentration group,and SOD activity obviously below than these groups(P<0.01,0.05).Blumea megacephala high concentration group the TP obviously higher than other groups,and SOD activity obviously below than other groups(P<0.01).Blumea megacephala medium concentration group and high concentration group the AST are no significant difference,but obviously below than other groups(P<0.01).Blumea megacephala high concentration group and Normal control group the MDA are no significant difference,but obviously below than other groups(P<0.01,0.05).Blumea megacephala medium concentration group the MDA obviously below than low concentration group(P<0.05).Model control group myocardial cell the number is significantly reduced,the capillary expansion between myocardial cells is obvious,and the area of cell degeneration necrosis is obvious.Nitroglycerine group,Blumea megacephala low and medium concentration groups the cardiomyocyte structure was better than that of the Model control group,the intercellular capillary dilation and denaturation necrosis of cardiac myocytes were all greatly improved.Blumea megacephala high concentration group yocardial cell structure is clear,there is no obvious degenerative necrosis and capillary dilation,it is close to the normal control group myocardial cell structure expression,and obviously excellent than Blumea megacephala low and high concentration groups.Conclusions Blumea megacephala have improve myocardial ischemia and hypoxia,intervention oxygen free radicals on myocardial cell injury,promotion of cardiomyocyte repair;These effects have positively correlated in the dosage and effectof.