活性维生素D联合唑来膦酸可以促进成骨细胞的增殖和分化

Active Vitamin D Combined with Zoledronic Acid can Promote the Proliferation and Differentiation of Osteoblasts

因骨量减少、骨微观结构退化导致的骨质疏松是中老年人常见疾病,主要表现在髋部和腰椎骨折,其中女性尤为严重。从细胞水平探究活性维生素D联合唑来膦酸在治疗老年骨质疏松性髋骨部骨折中的作用机理。本研究主要观察活性维生素D和唑来膦酸不同处理方式对Wistar大鼠原代培养成骨细胞增殖、分化、分泌能力及蛋白合成能力的影响,以探讨两者联用对骨骼的作用机制。活性维生素D联用唑来膦酸对成骨细胞增殖有明显促进作用;与空白对照组相比,活性维生素D与唑来膦酸联用能够显著提高成骨细胞蛋白激酶(PKC)、碱性磷酸酶(ALP)和骨钙素表达量。活性维生素D联用唑来膦酸对成骨细胞的调控作用可能与跨膜蛋白PKC信号转导有关,并介导碱性磷酸酶(ALP)和骨钙素高表达,从而促进成骨细胞的增殖、分化以及骨基质形成。

Osteoporosis due to reduced bone mass and deterioration of bone microstructure is a common disease in middle-aged and elderly people,mainly in hip and lumbar fractures,in which women are particularly affected.To investigate the mechanism of action of active vitamin D combined with zoledronic acid in the treatment of elderly osteoporotic hip fracture at the cellular level.The effects of different treatments of active vitamin D and zoledronic acid on the proliferation,differentiation,secretion and protein synthesis of primary cultured osteoblasts in Wistar rats were observed.Then the mechanism of bone formation when the two drugs are combined is studied.The cell growth curve showed that active vitamin D combined with zoledronic acid promoted the proliferation of osteoblasts.Compared with the blank control group,active vitamin D combined with zoledronic acid significantly increased osteoblast protein kinase.(PKC),alkaline phosphatase(ALP)and osteo-calcin expression levels.The regulation of active vitamin D combined with zoledronic acid on osteoblasts may be related to transmembrane protein PKC signal transduction and mediate high expression of alkaline phosphatase(ALP)and osteocalcin.Thereby promoting the proliferation,differentiation and bone matrix formation of osteoblasts.