抗阻运动对慢性癫痫大鼠癫痫发作和记忆缺陷的影响

Effects of Resistance Exercise on Seizures and Memory Deficits in Rats with Chronic Epilepsy

为了揭示抗阻运动对慢性癫痫大鼠癫痫发作和记忆缺陷的影响,本研究将雄性Wistar大鼠随机分为4组,即抗阻运动癫痫组(REG)、抗阻运动对照组(RCG)、癫痫组(EG)和对照组(CG),每组20只。通过腹腔注射毛果芸香碱诱导癫痫,然后REG组和RCG组大鼠进行4周的抗阻运动,记录各组大鼠的癫痫发作次数,并记录抑制性回避试验中大鼠进入黑色盒子的潜伏期,检测各组大鼠海马的IGF-1、BDNF、CREB、ERK和mTOR水平。研究显示,抗阻运动后,EG组的癫痫发作次数显著高于REG组。REG组的潜伏期显著高于EG组。抗阻运动后,EG组大鼠海马IGF-1蛋白水平显著高于REG组。此外,EG组大鼠海马BDNF蛋白水平显著低于REG组。REG组的ERK和mTOR磷酸化水平显著高于EG组。4组大鼠海马总CREB表达水平及CREB磷酸化水平均无显著差异。本研究表明,抗阻运动可有效降低癫痫大鼠的癫痫发作次数,并提高大鼠记忆力,减轻癫痫引起的记忆缺陷。抗阻运动抑制了癫痫诱导的IGF-1的上调,促进了BDNF的上调,并促进了与细胞增殖、生长、分化和存活相关的ERK和mTOR蛋白的活化。

To reveal the effects of resistance exercise on seizures and memory deficits in rats with chronic epilepsy,in this study,male Wistar rats were randomly divided into 4 groups,namely,resistance exercise epilepsy group(REG),resistance exercise control group(RCG),epilepsy group(EG)and control group(CG),with 20 rats in each group.Epilepsy was induced by intraperitoneal injection of pilocarpine,and then the rats in the REG group and the RCG group were subjected to resistance exercise for 4 weeks.The number of seizures in each group was recorded,and the latency of the rats entering the black box in the inhibitory avoidance test was recorded.The levels of IGF-1,BDNF,CREB,ERK and mTOR in the hippocampus of rats were detected.Studies have shown that after resistance exercise,the number of seizures in the EG group was significantly higher than that of the REG group.The latency of the REG group was significantly higher than that of the EG group.After resistance exercise,the level of IGF-1 protein in hippocampus of EG group was significantly higher than that of REG group.In addition,the level of BDNF protein in hippocampus of EG group was significantly lower than that of REG group.The ERK and mTOR phosphorylation levels in the REG group were significantly higher than those in the EG group.There was no significant difference in the expression of total CREB and CREB phosphorylation in hippocampus of the 4 groups of rats.This study shows that resistance exercise can effectively reduce the number of seizures in epileptic rats,improve memory and reduce memory deficits caused by epilepsy.Resistance exercise inhibits epileptically induced upregulation of IGF-1,promotes upregulation of BDNF,and promotes activation of ERK and mTOR proteins associated with cell proliferation,growth,differentiation,and survival.