摘要
为研究酵母细胞壁、紫外线(UV)照射以及二者共同作用对玉米黄曲霉毒素B1(AFB1)的脱毒效果,采用单因素设计,分为10个处理组,分别为对照组,每5 g发霉玉米中添加2、1、0.5、0.1 g酵母细胞壁的4个试验组,4个试验组置于紫外线照射组以及仅紫外线照射组,每组4个重复,所有试验组均于室温下放置3 d,采用酶联免疫法(ELISA)检测AFB1的含量。结果表明:不同添加量酵母细胞壁均对AFB1含量有影响,1 g时脱毒率最高,为(29.25±2.22)%,显著高于添加酵母细胞壁经紫外线照射组以及仅紫外线照射组(P<0.05),各酵母细胞壁组间差异不显著(P>0.05);紫外线照射对AFB1也有一定脱毒作用,但酵母细胞壁在紫外线照射下脱毒效果降低。
The objective of this study was to investigate the detoxification effects of yeast cell wall,ultraviolet(UV)radiation and their interaction detoxification for aflatoxin B1(AFB1)in mycotoxin-contaminated maize.The experiment was divided into 10 groups with 4 replicates in each according to single factor design,control croup,the yeast cell wall was added to the mycotoxin-contaminated maize with the levels of 2,1,0.5 g and 0.1 g,respectively,which were exposed to ultraviolet radiation,respectively,and only the UV-irradiated group,all the treatments were placed at room temperature for 3 days,the concentration of AFB1 was determined by ELISA.The results showed that adding 1 g yeast cell wall group was found to have the best detoxification effect,and the detoxification rate was(29.25±2.22)%,which was significantly higher than those of adding yeast cell exposed to ultraviolet radiation and only UV-irradiated group(P<0.05),the difference was not found among the adding yeast cell wall groups(P>0.05).Ultraviolet radiation also decreased the concentration of AFB1,however,the detoxification effects of adding yeast cell wall groups exposed to ultraviolet irradiation for AFB1 were reduced.
作者
王东旭
吴佳遥
梁鹏志
李留安
焦小丽
WANG Dongxu;WU Jiayao;LIANG Pengzhi;LI Liuan;JIAO Xiaoli(Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin Xiqing 300384,China)
出处
《中国饲料》
北大核心
2019年第23期88-90,共3页
China Feed
基金
天津市“131”创新型人才团队-“天津农学院功能性畜禽产品研发创新团队”建设项目(20180318)
天津市科技重大专项与工程项目“蛋鸡健康养殖和蛋品质质量综合提升工程技术集成与示范”(18ZXBFNC00310)