卷柏内生真菌多样性研究

Diversity of endophytic fungi isolated from Selaginella tamariscina

为探究蕨类植物内生真菌的多样性,本研究采用可培养方法分离江西龙虎山和广东丹霞山的岩壁常见蕨类植物——卷柏的内生真菌,结合形态特征和ITS序列进行菌株鉴定、研究多样性。结果表明:从1080个组织块中,共分离出234株菌株,卷柏内生真菌的总定殖率为18.98%,总分离率为21.67%。根据分离频率,毛壳菌属Chaetomium(26.50%)、青霉属Penicillium(18.38%)、棒束孢属Isaria(11.97%)、曲霉属Aspergillus(9.42%)为优势属。不同居群地春季卷柏的内生真菌多样性存在差异,龙虎山的分离率为34.72%,而丹霞山仅为6.39%,其优势属也不尽相同。对龙虎山不同季节的卷柏内生真菌多样性研究表明,春季的分离率为34.72%,高于冬季的23.89%,春、冬两季之间的真菌类群相似性系数仅为0.39,差异显著。研究结果将有助于进一步揭示丹霞地貌地区蕨类植物内生真菌物种组成及其影响因素。

The diversity of endophytic fungi in Selaginella tamariscina,a common fern on rock wall,from Longhu Mountain in Jiangxi Province and Danxia Mountain in Guangdong Province was explored.The fungi were isolated from 1080 tissue segments by culture-dependent method and identified at species or genus level based on morphological characteristics and molecular analysis.Among 234 isolates,the isolation frequency of Chaetomium,Penicillium,Isaria and Aspergillus were 26.50%,18.38%,11.97%and 9.42%,respectively.The diversity of endophytic fungi in S.tamariscina was notably distinct in different habitats in spring.The isolation frequency from Longhu Mountain was 34.72%,but that from Danxia Mountain only 6.39%.The dominant genera also varied with different locations.The endophytic fungal diversity changed with different seasons.The investigation results in Longhu Mountain showed that the isolation frequency of the fungi in spring was 34.72%,and that in winter 23.89%and the similarity coefficient of the fungi between spring and winter was 0.39,indicating that the community composition and dominant genera of endophytic fungi markedly differed between seasons.This study provides references for further study on endophytic fungi of pteridophytes in Danxia landform.