迷走神经途径的L-谷氨酸与十二指肠运动的量效关系

Dose-effect relationship between L-glutamic acid and duodenal movement in the vagus pathway

目的 构建经迷走神经背核(DMV)注射L-谷氨酸(L-Glu)的剂量与雌性家兔十二指肠肌电活动指数的数学模型,为探讨激素、细胞因子等影响迷走神经活动的作用机制提供实验依据。方法 采用DMV显微注射技术,分别按0 mol/L、0. 05 mol/L、0. 10 mol/L、0. 15 mol/L和0. 20 mol/L剂量经DMV注射L-Glu,每剂量组样本数为5只,监测卵巢摘除家兔的十二指肠肌电活动;并构建L-Glu的剂量与十二指肠肌电活动指数之间的数学模型。结果 经t检验分析,相邻剂量组之间,肌电活动的振幅逐渐增强,且差异极显著(P<0. 01);频率逐渐加强,其中0mol/L与0. 05 mol/L、0. 10 mol/L与0. 15 mol/L组间差异有显著性(P<0. 05);肌电活动指数逐渐增强,且组间差异极显著(P<0. 01)。以L-Glu浓度为自变量x,十二指肠肌电活动指数为因变量y,构建的数学模型的函数关系式为:y=13. 71/1+exp [-22. 35×(x-0. 082)],函数拟合精度:R2=0. 9948,P<0. 01,属于增长型S型逻辑函数。函数分析表明,0. 082 mol/L L-Glu剂量为该逻辑函数的拐点值;在L-Glu剂量范围为0 mol/L^0. 082 mol/L时,十二指肠肌电活动指数成指数型增长模式;当L-Glu剂量大于0. 082 mol/L时,十二指肠肌电活动指数呈对数型增长模式,且其理论极限值为13. 71。结论 L-Glu通过DMV对家兔十二指肠肌电活动的促进作用具有显著的量效关系,且具有区间作用的特点。

Objective To establish a mathematical model of the injection by dorsal motor nucleus of vagus(DMV),the dose of L-glutamic acid(L-Glu)and the duodenal myoelectric activity index of female rabbits was established to provide a theoretical basis for studying the mechanism of action of hormones,cytokines and other vagal activity.Methods In this experiment,we used microinjection technique to inject L-Glu to DMV by doses of 0 mol/L,0.05 mol/L,0.10 mol/L,0.15 mol/L and 0.20 mol/L,monitoring the duodenal myoelectric activity of ovarian abducted rabbits,the number of samples per dose group was 5,and construct a mathematical model between the dose of L-Glu and the index of duodenal myoelectric activity.Results After t-test analysis,the amplitude of myoelectric activity increased gradually between adjacent dose groups,and the difference was extremely significant(P<0.01).The frequency was gradually strengthened,and the difference between 0 mol/L and 0.05 mol/L,0.10 mol/L and 0.15 mol/L was significant(P<0.05).The index of myoelectric activity increased gradually,and the difference between groups was extremely significant(P<0.01).With L-Glu concentration as the independent variable x,the duodenal myoelectric activity index was the dependent variable y,the constructor relationship was y=13.71/1+exp[-22.35 ×(x-0.082)],function fitting accuracy was R2=0.9948,P<0.01,belonging to growth type S logic function.Function analysis showed that the L-Glu dose of 0.082 mol/L was the inflection point of the logic function.As the dose range of L-Glu was 0 mol/L-0.082 mol/L,duodenal myoelectric activity index showed an exponential growth pattern,as the L-Glu dose was greater than 0.082 mol/L,the duodenal myoelectric activity index showed a logarithmic growth pattern with a theoretical limit of 13.71.Conclusion L-Glu has a significant dose-effect relationship on the promotion of duodenal myoelectric activity in rabbits by DM V,and have the effect of interval,the mathematical model laid the theoretical foundation for further research on the role of hormones and cytokines on this basis.