宁夏地区非小细胞肺癌EML4-ALK融合基因的表达特征分析

Analysis of expression characteristics of EML4-ALK fusion gene in non-small cell lung cancer in Ningxia

目的探讨宁夏地区NSCLC患者EML4-ALK基因的表达分布情况及克唑替尼靶向治疗的效果。方法RT-PCR检测EML4-ALK基因的表达,统计分析临床病理特征、靶向治疗与阳性表达的相关性。结果EML4-ALK基因阳性表达率12.5%,年龄<55岁、不吸烟、临床高分期和腺癌与对照组相比差异无统计学意义(P>0.05),女性组与男性组相比差异显著(P<0.05);EML4-ALK基因阳性的患者克唑替尼靶向治疗后中位总生存时间为8个月(95%CI,7~12个月);阴性的患者中位总生存时间为9个月(95%CI,5~13个月),2组无差异(P>0.05)。结论宁夏地区NSCLC患者EML4-ALK融合基因与年龄、吸烟、临床分期高、腺癌类型无关,克唑替尼靶向治疗EML4-ALK阳性女性患者具有一定疗效。

Objective To investigate the expression and distribution of EML4-ALK fusion gene in NSCLC patients in Ningxia and the effect of clozapine targeted therapy.Methods The expression of EML4-ALK fusion gene were identified by RT-PCR.The correlation among the clinicopathological characteristics,targeted therapy and the expression of EML4-ALK fusion gene of NSCLC patients in Ningxia were analyzed.Results The positive expression rate of EML4-ALK fusion gene was 6.25%.There was no significant difference in age(less than 55),non-smoking,clinical high stage and adenocarcinoma compared with the control group(P<0.05).There was significant difference between the female group and the male group(P<0.05).The median survival time of patients with positive EML4-ALK fusion gene after treated with clozapine was 8 months(95%CI,7~12 months).The median survival time of patients with negative expression was 9 months(95%CI,5~13 months).There was no difference between the two groups.Conclusion The EML4-ALK fusion gene of NSCLC patients in Ningxia is not reIated to age,smoking,high clinical stage and type of adenocarcinoma,but related to the gender.Moreover,targeted therapy with crizotinib in female patients with EML4-ALK positive is effective.