核糖体蛋白RPL34-PS1对B细胞淋巴瘤的生长促进作用

Effect of Ribosomal Protein RPL34-PS1 on the Growth of B-cell Lymphoma

一种核糖体蛋白RPL34具有促进B细胞淋巴瘤生长的作用,通过分子克隆将RPL34基因的序列克隆到逆转录病毒载体pBABE-Puro上后,包装逆转录病毒并感染Balb/c小鼠来源的B淋巴瘤细胞38B9,经嘌呤霉素筛选后构建稳定过表达RPL34的B淋巴瘤细胞系RPL34-38B9。体外培养计数比较细胞增殖变化情况;流式检测细胞凋亡和细胞周期水平;小鼠皮下荷瘤并测量肿瘤体积。结果表明:过表达的核糖体蛋白RPL34能够显著促进B淋巴瘤细胞的生长速率,减少细胞凋亡,同时能够显著增加B淋巴瘤细胞的成瘤速率。

A ribosomal protein RPL34 has the effect of promoting the growth of B-cell lymphoma.After cloning the sequence of RPL34 gene onto the retrovirus vector pbab-puro by molecular cloning,the retrovirus was packaged and infected with Balb/c murine derived B lymphoma cell 38B9.After screening with purinomycin,a stable B lymphoma cell line rpl34-38b9 overexpressing RPL34 was constructed.Cell proliferation changes were compared in vitro culture count.Cell apoptosis and cell cycle were determined by flow cytometry.Subcutaneous tumor is borne in mice and the tumor volume is measured.The results showed that the overexpressed ribosomal protein RPL34 could significantly promote the growth rate of B lymphoma cells,reduce apoptosis,and significantly increase the tumorigenic rate of B lymphoma cells.