铁皮石斛及其冻干粉对免疫抑制小鼠的免疫调节作用差异研究

Study on the Difference of Immunomodulatory Effects of Dendrobium officinale and Its Lyophilized Power on Immunosuppressed Mice

[目的]探讨铁皮石斛及其冻干粉对提升免疫抑制小鼠的免疫调节作用差异。[方法]120只SPF级昆明雄性小鼠分为空白对照组、环磷酰胺模型组、铁皮石斛组和铁皮石斛冻干粉组。两组给药组连续灌胃30 d后,24 h内模型组、铁皮石斛及其冻干粉组一次性腹腔注射200 mg/kg的环磷酰胺免疫抑制造模。试验结束后测定胸腺系数、脾脏系数、足趾肿胀度,以及血清中的细胞因子IFN-γ、IL-2和IL-6的含量。[结果]铁皮石斛及其冻干粉均可以升高免疫抑制小鼠的胸腺和脾脏系数;增加免疫抑制小鼠的足趾肿胀度;上调免疫抑制小鼠血清中TNF-α、IFN-γ、IL-2和IL-6的水平。[结论]铁皮石斛及其冻干粉对环磷酰胺引起的免疫抑制具有明显的改善作用,且后者药效更好。

[Objective]The research aimed to study the difference in immunomodulatory effects of Dendrobium officinale and its lyophilized power on immunosuppressed mice.[Method]120 SPF Kunming male mice were divided into control group,cyclophosphamide modle group,Dendrobium officinale and its lyophilized power group.Dendrobium officinale and its lyophilized power groups were continuously administrated for 30 days.At 24 hours before the end of the experiment,the model group,Dendrobium officinale and its lyophilized power group were intraperitoneally injected with 200 mg/kg cyclophosphamide.At the end of the experiment,the thymus index,spleen index,DTH,and serum cytokines IFN-γ,IL-2 and IL-6 were measured.[Result]Dendrobium officinale and its lyophilized power increased the thymus index,spleen index and toe swelling,up-regulated the levels of IFN-γ,IL-2 and IL-6 in the serum of immunosuppressed mice.[Conclusion]Dendrobium officinale and its lyophilized power has a significant improvement on the immunosuppression caused by cyclophosphamide,and the latter is better.