载麦冬皂苷的明胶微球对成骨细胞的调控作用

Regulation effects of ophiopogonin-loaded gelatin microspheres on osteoblasts

以麦冬甾体皂苷为模型药物,以明胶为载体原料制备载药微球,并研究其对成骨细胞的调控作用。首先采用双乳化-化学交联技术构建载药明胶微球,再对其释药特点、调控成骨细胞生理功能的效应等方面开展研究。结果发现构建的载药微球不仅有效缓解药物突释现象并延长药物释放时间,而且可以有效刺激成骨细胞增殖、碱性磷酸酶分泌及矿化,促其向骨组织分化。

Ophiopogonin was used as the model drug and gelatin as the carrier material to prepare drug-loaded microspheres, whose regulatory effects on osteoblasts were studied. Firstly, the drug-loaded gelatin microspheres were constructed by double emulsification-chemical crosslinking technology, and then the drug release characteristics and the effects on regulating the physiological function of osteoblasts were studied. Results show that the microspheres could not only effectively alleviate drug burst release and prolong its release time, but also stimulate the proliferation of osteoblasts, and the secretion and mineralization of alkaline phosphatase, and promote cell differentiation towards bone tissue.