五味子果肉和种子精油的化学成分与生物活性对比研究（英文）

Comparative Study on Essential Oils of Schisandra chinensis Fruits Pulp and Seeds: Chemical Composition and Bioactivities

为明确五味子果肉和种子精油成分和生物活性的异同,通过气质联用仪对五味子果肉精油(PEO)和种子精油(SEO)进行成分分析,还对2种精油的抗肿瘤活性、抑菌活性和体外抗氧化活性等生物活性进行了对比研究。分析结果显示:从PEO和SEO中分别鉴定出55种和45种成分,质量分数分别占精油总量94.04%和93.39%。抗肿瘤活性显示:PEO和SEO对HeLa和BGC-823肿瘤细胞均具有较好的抗肿瘤活性(IC50值为39.07~56.43 mg/L),但2种精油对A549细胞均不显示抗肿瘤活性。抑菌活性检测结果表明:PEO和SEO对金黄色葡萄球菌、枯草芽孢杆菌、绿脓杆菌和大肠杆菌等4种细菌生长均具有一定抑制作用,PEO的MIC值为245.00~504.33 mg/L,SEO的MIC值为495.00~1 004.68 mg/L。此外,实验结果还显示PEO较SEO有更好的体外抗氧化活性,其中PEO对DPPH·和ABTS+·的IC50值分别为3.15和2.31 g/L,SEO对DPPH·和ABTS+·的IC50值分别为5.43和10.11 g/L。

In order to investigate the differences and similarities between the essential oils( EOs) obtained from Schisandra chinensis fruits pulp and seeds( PEO and SEO),the chemical composition,anti-tumor property,antimicrobial and antioxidant activity were examined. According to the results of GC-MS( gas chromatography-mass spectrometry,GC-MS) analyses,55 and 45 compounds which represented 94. 04% and 93. 39% of the oils were identified,respectively. In addition,anti-tumor activity of essential oils was carried out using the Sulforhodamine B( SRB) method. The oils also revealed the highest anti-tumor activity against He La and BGC-823 cells,but they had no effect on A549 cells. They presented IC50 values from 39. 07 mg/L to 56. 43 mg/L. PEO and SEO also exhibited inhibitory activity against four bacterial strains including Staphylococcus aureus,Bacillus subtilis,Pseudomonas aeruginosa and Escherichia coli. MIC of PEO were 245. 00-504. 33 mg/L,MIC of SEO were 495. 00-1 004. 68 mg/L. Furthermore,in all assays of antioxidant ability,PEO showed stronger antioxidant properties than SEO. IC50 value of PEO against DPPH and ABTS radical were 3. 15 and 2. 31 g/L,respectively. IC50 of SEO against DPPH and ABTS radical were 5. 43 and 10. 11 g/L,respectively. In a word,this study supports the potentials of PEO and SEO as antibacterial and antineoplastic agent.