文昌鸡白麻羽性状与SLC45A2多态性关系

Relationship between the Trait of White-partridge Feathers and SLC45A2 Polymorphism in Wenchang Chicken

【目的】研究文昌鸡白麻羽性状的遗传规律,鉴定与白麻羽性状相关的基因变异,为文昌鸡白麻羽群体的利用以及白麻羽性状的分子标记辅助选择提供参考。【方法】利用正反交试验探索白麻羽性状的遗传规律;根据白麻羽性状的特点结合前人研究结果,选取SLC45A2基因为候选基因,利用重测序的方法筛选外显子区域内的变异;并重点验证外显子区域的错义突变和无义突变与白麻羽性状的关系。【结果】黄麻羽个体与白麻羽个体正反交试验结果表明,白麻羽性状相对于黄麻羽呈伴性隐性遗传。试验以白麻羽和黄麻个体为DNA模板,扩增了SLC45A2上的7个外显子区域,结果共筛选到5个SNP,其中3个位于外显子上,2个为错义突变(c.a74g和c.g1154c),1个为同义突变(c.c561t)。以文昌鸡白麻羽群体的文昌鸡和非白麻羽性状的文昌鸡、霸王山鸡、清远麻鸡、杏花鸡、红色原鸡为材料,验证上述错义突变,结果显示c.g1154c位点与白麻羽性状完全连锁。【结论】白麻羽性状呈现伴性隐性遗传,c.g1154c可能是白麻羽性状的致因突变。

【Objective】In this study,the inheritance of the trait of white-partridge feathers in Wenchang chicken was studied and the related genetic variation was identified.,which provided references for the utilization of the white-partridge population and molecular marker-assisted selection of white-partridge feather trait.【Method】Firstly,the inheritance of white-partridge feather trait was studied by reciprocal test.Then,SLC45A2 was selected as a candidate gene based on the genetic characteristics of white-partridge feather and results of previous researches.The variation in the exon domain of SLC45A2 gene was analyzed by using re-sequencing method.Lastly,the relationship between missense mutation/nonsense mutation in the exon domain and the trait of white-partridge feather was verified.【Result】The results of reciprocal test showed that the trait of white-partridge feather was recessive inheritance compared with that of yellow-partridge feather.The amplification results indicated that there were five SNPs in SLC45A2 gene,in which three were located in exon domain,including two were missense mutation(c.a74g and c.g1154c)and one was synonymous mutation(c.c561t).Finally,the result showed that C.G1154C site of SLC45A2 gene was completely linked with the white-partridge feather trait based on missense mutation study in Wenchang chicken,Bawang chicken,Qingyuan partridge chicken,Xinghua chicken and Gallus gallus.【Conclusion】The trait of white-partridge feathers showed recessive inheritance and c.g1154c site might be the causation.