甘蓝型油菜多主花序性状的表型特点与遗传分析

Phenotypic characteristics and genetic analysis of multi-inflorescence trait in Brassica napus

为改良油菜株型以提高产量和适应机械化,分析多主花序油菜的表型特点和遗传规律,利用1个甘蓝型油菜多主花序突变体和1个单主花序普通材料,通过扫描电镜观察甘蓝型油菜多主花序材料顶端分生组织发育过程,认为多主花序性状的形成与顶端分生组织的异常发育有关。对F1、RF1以及分离群体做表型鉴定,结果表明:多主花序性状在两个供试亲本材料间存在1对显性基因的差异,无细胞质效应。利用油菜60K SNP芯片结合分子标记技术,初步构建多主花序基因MI的遗传连锁图谱,两侧最近标记L04-2和L06-14与目标基因的遗传距离分别为4.4 cM和9.3 cM,物理距离为2.5 Mb。

For plant type breeding, a multi-inflorescence rapeseed mutant was investigated on phenotype and molecular markers. Results of F1, RF1 and segregation population indicated that one pair of dominant genes were different between the 2 tested parental materials and no cytoplasmic effect was detected. Using Brassica napus 60 K SNP array combined with molecular marker technology, a linkage map of the multi-inflorescence gene MI was constructed. Two flanking markers L04-2 and L06-14 were mapped at 4.4 cM and 9.3 cM from MI gene respectively.Their physical distance was 2.5 Mb.