高效液相色谱法同时快速测定植物油中的三种抗氧化剂

Simultaneous and rapid determination of three antioxidants in vegetable oils by high performance liquid chromatography

建立了同时快速测定植物油中特丁基对苯二酚(TBHQ),叔丁基对羟基茴香醚(BHA),2,6-二叔丁基对甲酚(BHT)3种抗氧化剂的反相高效液相色谱方法。样品中的抗氧化剂采用甲醇直接提取,供高效液相色谱测定,采用Zorbax ODS色谱柱(250 mm×4.6 mm,5μm),流动相为水和甲醇,梯度洗脱,柱温35℃,流速1.0 mL/min,波长为280 nm。在5.0~100.0μg/mL的线性范围内,3种抗氧化剂的相关系数均大于0.999,0.04、0.1、0.25 g/kg 3个加标水平的相对标准偏差均小于2.0%,在7种常见的植物油中TBHQ、BHA和BHT的回收率分别为94.01~98.60%、96.30~98.35%和91.67~102.34%,检出限分别为0.65、0.46、0.87 mg/kg。该方法适用于常见植物油样品中3种抗氧化剂的同时快速测定。

A method of simultaneous and rapid determination of 3 antioxidants in vegetable oils,including tertiary butylhydroquinone(TBHQ),butylated hydroxyanisol(BHA)and butylated hydroxytoluene(BHT)by reversed phase high performance liquid chromatography was established.Antioxidants in samples were extracted directly with methanol,and an HPLC method was applied with a Zorbax ODS column(250 mm×4.6 mm,5μm)by a gradient elution using water and methanol as the mobile phase.The flow rate was 1.0 mL/min and the column temperature was 35℃.The detective wavelength was 280 nm.The linear range of three antioxidants was 5.0~100.0μg/mL.The correlation coefficients for the regression equation were all higher than 0.999,and the relative standard deviations were all less than 2.0%at the spiked levels of 0.04,0.1,0.25 g/kg.The recovery rates for TBHQ,BHA and BHT in 7 common vegetable oils were 94.01~98.60%,96.30~98.35%and 91.67~102.34%,and the detection limits were 0.65,0.46,0.87 mg/kg,respectively.This method is suitable for simultaneous and rapid determination of three antioxidants in common vegetable oil samples.