谷子矮秆突变体d93090的表型变异及其对赤霉素的敏感性分析

The Phenotypic Variations and GA Sensitivity of a Dwarf Mutant d93090 in Foxtail Millet

谷子矮秆突变体93090(暂命名为d93090)是野生型高秆谷子品系93090经60Co-γ辐射诱变获得的,本研究对其矮化表型及其对赤霉素的敏感性进行了分析。结果表明,d93090株高约为野生型的60%左右,叶色变深、茎秆稍倾斜、茎节数不变、花期较对照推迟3~5d;幼苗期的苗长、第二叶鞘长和胚轴长均对外源GA3敏感,拔节期喷施外源GA3,d93090的株高部分恢复;d93090内源GA1含量显著低于野生型。d93090突变体是个半矮秆的突变类型,为谷子矮化育种提供了新材料,其矮秆性与GA生物合成途径相关。

A stably inherited dwarf mutant d93090 was obtained from a wild type tall line 93090 in foxtail millet induced by 60Co-γ irradiation.Then its dwarfing morphology and the characteristics response to gibberellin were analyzed.The results showed:Compared with the wild type,the plant height of the dwarf mutant was about 60%of that of the wild type.And other features,such as darker leaves in color,slightly sloping stem,the same stem nodes and delayed flowering time of about 3-5 days were also observed.The seedling length,the length of second leaf sheath and hypocotyl length at seedling stage were sensitive to exogenous GA3.While exogenous GA3 was sprayed at jointing stage,plant height of dwarf mutant was partially restored.The content of endogenous GA1 in dwarf mutant was significantly lower than that in wild type.It can be concluded this mutant is a semi-dwarf mutant and is related to the GA metabolic pathway.This study provides new germplasm for dwarf breeding in foxtail millet.