SiASRs家族基因的鉴定及表达分析

Identification and Expression Analysis of ASR Family Genes in Setaria italica

ASR蛋白是植物特有的一类蛋白,是参与并提高植物抗旱性和耐盐性过程中非常重要的蛋白之一。利用生物信息学方法,对8个谷子SLISRs家族基因编码的蛋白进行理化性质的分析、启动子的分析和系统进化树构建等,并结合qRT-PCR进行10%PEG-6000和150mmol/L NaCl胁迫下的表达分析。结果表明,谷子SiASRs家族的8个基因编码的蛋白都包含典型的ABA/WDS结构,该基因家族编码的蛋白质都不具有信号肽。系统发育树分析结果表明,在遗传进化上谷子与柳枝稷亲缘关系较近,与双子叶的番茄和大豆ASR蛋白的亲缘关系较远。PEG胁迫下基因的表达分析显示,8个SiASRs基因在受到PEG胁迫诱导后在根、茎、叶中表达量差异显著。NaCl胁迫下基因的表达分析结果显示,8个ASR基因对NaCl胁迫的响应主要在叶中体现,整体呈上调表达趋势;8个ASR基因在茎中表达变化较为稳定;在根部主要由ASR1,ASR2、ASR4,ASR5,ASR6参与NaCl胁迫响应,均为上调表达。

ASR protein is a plant-specific protein,which it is one of the most important proteins involved in the drought resistance and salt tolerance of plants.Bioinformatics methods were used to analyze the physicochemical properties of proteins,promoter analysis and phylogenetic tree construction of eight SiASRs family genes in millet.PEG-6000(10%)and salt(150mmol/L)stresses were arranged for study expression using qRT-PCR.The proteins encoded by the 8 millet SiASRs family genes contained typical ABA/WDS structures,and no signal peptides.Phylogenetic tree showed that the genetic relationship between millet and switchgrass was closer.The evolution among millet ASR protein and tomato ASR protein and soybean ASR protein was relatively far.The results of expression analysis under PEG stress showed that the expression of 8 SiASR was significantly different in different tissues after induction by drought stress.The results of expression analysis under salt stress showed that the responses of 8 ASR genes to salt stress were mainly reflected in the leaves,and the overall expression was up?regulated.The expression of 8 ASR genes in stems was stable;In the roots,ASR1,ASR2,ASR4,ASR5 and ASR6 were involved in salt stress response,and all 5 genes were up?regulated.