Fas配体过表达对人牙周膜干细胞成骨分化的影响

Effects of Fas Ligand Overexpression on Osteogenic Differentiation Potential of Human PDLSCs

目的:探讨Fas配体(Fas ligand,FasL)过表达对人牙周膜干细胞(periodontal ligament stem cells,PDLSCs)骨向分化能力的影响。方法:体外有限稀释克隆化培养人PDLSCs,构建FasL过表达质粒,转染至PDLSCs,实验分为对照组(Control组)、空载体对照组(NC组)、FasL过表达组(EX组),对各组进行成骨诱导,茜素红染色、实时荧光定量PCR(Real-time PCR)和Western blot检测PDLSCs成骨分化的能力。结果:EX组成骨分化能力较Control组和NC组有明显升高(P<0.05),Control组和NC组比较差异无统计学意义(P>0.05)。结论:FasL可以促进PDLSCs成骨分化。

Objective:To investigate the effect of FasL overexpression on osteogenic differentiation of human periodontal ligament stem cells(PDLSCs).Methods:Human PDLSCs were cloned and cultured in vitro with limited dilution.FasL overexpression plasmid was constructed and transfected into PDLSCs.The experiment was divided into control group,no-load control group(NC group),and FasL overexpression group(EX group).Osteogenic was induced in each group.Alizarin red staining,real-time PCR,and western blot were used to detect the osteogenic differentiation ability of PDLSCs.Results:The osteogenic differentiation ability of EX group was significantly higher than that of control group and NC group(P<0.05).There was no significant difference between control group and NC group(P>0.05).Conclusion:FasL can promote the osteogenic differentiation of human PDLSCs.