短程硝化SBR实时控制与微生物群落研究

The study on real-time control strategy of short-cut nitrification SBR and microbial community

采用短程硝化—反硝化SBR处理模拟氨氮废水,通过调控pH、溶解氧(DO)、温度和曝气时间实现SBR快速启动,并用DO、ORP的一阶导数实时控制反应。结果表明:第20天亚硝化率(NAR)达到95%。采用一阶导数作为控制参数时,NH4^+-N去除率与NAR维持在97%以上。通过Slope公式对一阶导数算法进行优化,可达到更加稳定的控制效果。污泥的16S rDNA测序表明,Chloroflexi、Bacteroidetes、Proteobacteria和Actinobacteria是污泥中的优势菌门。在控制污泥龄(SRT)及实时控制条件下,NOB被逐渐淘洗出反应器。

A shortcut nitrification-denitrification SBR is developed to treat the synthetic ammonium nitrogen wastewater.SBR can be started rapidly by adjusting the pH,dissolved oxygen(DO),temperature and aeration time.Besides,first derivatives of DO and ORP are applied to control the reaction process in realtime.The experiment results show the nitrite accumulation rate(NAR)reaches more than 95%on day 20.As a result of the control of first derivative,the ammonia nitrogen removal efficiency and NAR are above 97%.After the first derivative algorithm is optimized by Slope formula,a more stable control effect could be achieved.The results of 16S rDNA sequencing of sludge show that Chloroflexi,Bacteroidetes,Proteobacteria and Actinobacteria are the dominant bacteria in the sludge.Under the control of sludge retention time(SRT)and realtime control strategy,NOB is gradually washed out of the reactor.