裸鼠玻璃体腔视网膜母细胞瘤移植瘤模型的建立

Estabishment of retinoblastoma implantation model in nude mice vitreous cavity

目的建立裸鼠玻璃体腔视网膜母细胞瘤移植瘤模型,观察移植瘤的生长特性,探索适用于视网膜母细胞瘤体内实验的建模方法。设计实验研究。研究对象六周龄雄性BALB/c裸鼠30只。方法30只BALB/c裸鼠随机分为三组,每组10只;体外培养Y79、WERI-RB1和SO-Rb50三株视网膜母细胞瘤细胞,调整细胞浓度为1.0×108个/ml;A组取Y79细胞悬液3μl注射于裸鼠右眼玻璃体腔,B组和C组分别注射WERI-RB1和SO-Rb50细胞,方法同A组。观察肿瘤生长侵袭情况。主要指标成瘤率、成瘤时间及肿瘤侵犯情况。结果三组BALB/c裸鼠右眼玻璃体腔注射肿瘤细胞后,A组成瘤率100%,成瘤时间3天~11周,平均成瘤时间(32.6±5.77)天;B组成瘤率88.9%,成瘤时间16天~14周,平均成瘤时间(57.4±8.42)天;C组成瘤率88.9%,成瘤时间19天~10周,平均成瘤时间(43.7±7.16)天;实验眼肿物经病理证实均为视网膜母细胞瘤。A组发生视神经侵犯7例,B组2例,C组6例;A组发生前房侵犯5例,B组无,C组4例;A组发生虹膜/睫状体侵犯5例,B组无,C组4例;三组均未见脉络膜侵犯;A组发生角膜新生血管3例,B组无,C组3例。结论通过BALB/c裸鼠玻璃体腔注射肿瘤细胞可建立视网膜母细胞瘤移植瘤模型,成瘤率高,Y79和SO-Rb50细胞株移植瘤的侵袭性高于WERI-RB1细胞株。

Objective To establish animal model of retinoblastoma by injecting the tumor cells into the vitreous cavity of nude mice and to observe growth status of the tumor.Design Experimental research.Participants Thirty BALB/c nude mice.Methods The cell suspension of human retinoblastoma cell line Y79,WERI-RB1 and SO-Rb50 were prepared,the cell concentration was adjusted to 1.0×108/ml,then 3μl of the tumor cell suspensions were injected into the right vitreous cavity of 10 nude mice in the each cell line group(group A,B,and C,respectively).Main Outcome Measures tumorigenic rate,tumorigenic time,and the growth status of the tumor.Results In Y79 cell line group(group A),all of the models of retinoblastoma were successfully established by injecting the cell suspensions of cells into the mice vitreous cavities.The growth cycle was 3 days to 10 weeks.In WERI-RB1 cell line group(group B),88.9%of the models were successfully established.The growth cycle was 16 days to 14 weeks.In SO-Rb50 cell line group(group C),88.9%of the models were successfully established.The growth cycle was 19 days to 10 weeks.There were 7 cases of optic nerve invasion in group A,2 cases in group B,and 6 cases in group C.There were 5 cases of anterior chamber invasion in group A,none in group B,and 4 cases in group C.There were 5 cases of iris/ciliary body invasion in group A,none in group B,and 4 cases in group C.No choroid invasion was found in the three groups.There were 3 cases of corneal neovascularization in group A,none in group B,and 3 cases in group C.Conclusion The animal model of retinoblastoma can be established by injection the tumor cells into vitreous cavities of the nude mice.Increased invasiveness was seen in Y79 and SO-Rb50 cell lines than WERI-RB1 cell lines.