脑肠肽对脓毒症大鼠胃液分泌及胃血流的影响

Effect of ghrelin on gastric juice secretion and gastric blood flow in septic rats

目的观察脑肠肽(Ghrelin)对脓毒症大鼠胃液分泌量、胃液pH值及胃血流的影响.方法将72只雄性Wistar大鼠按随机数字表法分为六组,正常对照组、假手术组、脓毒症组、Ghrelin组、促生长激素释放肽-6(GHRP-6)组及Obestatin组(每组12只),各组于造模成功并给予干预措施后测胃血流,并于干预后2、4、8、12 h分别处死3只大鼠,取胃,收集胃液测量胃液的pH值及胃液的体积.结果脓毒症组胃大弯处血流较正常对照组明显减少(284.31±95.66vs.338.13±4.70,P<0.05),Ghrelin组血流较脓毒症组明显增多(377.83±99.01 vs.284.31±95.66,P<0.05),Obestatin组血流较Ghrelin组明显减少(320.68±130.06 vs.377.83±99.01,P<0.05);正常对照组与假手术组各时间点pH值及胃液体积比较差异均无统计学意义(P>0.05).与假手术组比较,脓毒症组pH值在干预后4h起即明显升高(均P<0.05),胃液体积在干预后2、8、12h减小(均P<0.05).与假手术组和脓毒症组比较,Ghrelin组和GHRP组pH值在干预后4h起即明显降低(均P<0.05),Ghrelin组胃液体积在干预后2h即明显升高(均P<0.05).与Ghrelin组比较,GHRP组pH值仅在干预后8h时间点差异有统计学意义(P<0.05);GHRP-6组与假手术组、脓毒症组及Ghrelin组比较,胃液体积在干预后2h起即明显升高(P<0.05).Obestatin组与其余各组比较,pH值及胃液体积在各时间点差异均有统计学意义(均P<0.05).结论Ghrelin能够改善脓毒症大鼠胃血流,促进脓毒症大鼠胃液的分泌.

Objective To investigate the effects of ghrelin on gastric juice secretion and gastric juice pH in septic rats.Methods Seventy-two male Wistar rats were randomly divided into six groups:normal control,sham,sepsis,ghrelin group,growth hormone releasing peptide-6(GHRP-6)group,and obestatin group,every group have twelve rats.2 h,4 h,8 h and 12 h after the intervention,three rats were sacrificed and the gastric juice was collected to measure the pH value and volume.Results There was no significant difference in pH value and gastric fluid volume between control group and sham operation group(P>0.05).Compared with sham operation group,the pH value in sepsis group increased significantly at 4 h after intervention(all P>0.05),and the gastric fluid volume decreased at 2 h,8 h and 12 h after intervention(all P<0.05).Compared with sham operation group and sepsis group,the pH value of ghrelin group was significantly lower 4 h after intervention(all 0.05),and the gastric juice volume was significantly increased 2 h after intervention(all P<0.05).Compared with the normal control group,the pH value of GHRP-6 group decreased significantly 2 h after intervention(P<0.05),and decreased significantly 4 hours after intervention(P<0.05).Compared with the sepsis group,the pH value of GHRP-6 group decreased significantly 4 h after intervention(P<0.05).Compared with ghrelin group,the difference was only 8 h after intervention(P<0.05).The difference was statistically significant(P<0.05).Compared with sham operation group,sepsis group and ghrelin group,the volume of gastric juice in GHRP-6 group increased significantly 2 h after intervention(P<0.05).There were significant differences in pH value and gastric fluid volume between obestatin group and other groups(P<0.05).Conclusion Ghrelin can promote the secretion of gastric juice in septic rats.