仿刺参幼参对饲料中维生素B6需求量的研究

Dietary vitamin B6 requirement of juvenile sea cucumber（Apostichopus japonicus）

为研究仿刺参幼参对维生素B6的最适需求量,配制维生素B6实测含量分别为1.23、 5.29、9.35、17.47、33.71和66.17 mg/kg的6组实验饲料D1、D2、D3、D4、D5和D6组, 饲喂初始体质量为(12.23±0.11) g的仿刺参幼参12周。结果显示,①随着维生素B6含量的 增加,实验仿刺参的增重率、特定生长率均先升后降,在D5组达到最高值;体壁粗蛋白 含量先升后降,D6组显著低于其他组;D1组粗脂肪含量显著低于其他组;②体腔液 中葡萄糖-6-磷酸脱氢酶、异柠檬酸脱氢酶、乙酰辅酶A羧化酶及一氧化氮合酶活性均先 升后降,D6组显著低于其他组;③随着饲料中维生素B6含量的增加,肠道蛋白酶及淀 粉酶活性显著升高,纤维素酶活性显著降低,肠壁厚度及绒毛长度均显著升高。以增重 率为评价指标,经一元二次回归分析得出仿刺参幼参饲料中维生素B6的适宜需求量为 45 mg/kg。

The present trial was conducted to investigate the dietary vitamin B6 requirement of sea cucumber Apostichopus japonicas Selenka juveniles. The experimental diets were formulated with graded levels of vitamin B6 (1.23, 5.29, 9.35, 17.47, 33.71, 66.17 mg/kg), and named D1, D2, D3, D4, D5 and D6 groups. Each diet was randomly assigned with triplicate sea cucumber juveniles with initial body weight (12.23±0.11) g for 12 weeks. The results showed that weight gain rate and specific growth rate presented increasing first and then decreasing afterwards, and got maximum in D5 group. Crude protein of body wall was increased first with dietary vitamin B6 and then decreased, and crude protein of D6 group was significantly lower than other groups;Crude lipid of D1 group was significantly lower than other groups. All of activities of glucose 6-phosphatede hydrogenase, isocitrate dehydrogenase, acetyl coA carboxylase, and nitric oxide synthesis were increased first and then decreased afterwards, activity of D6 group was significantly lower than other groups. With the increasing of dietary vitamin B6, both protease and amylase were increased, and cellulase was decreased with dietary vitamin B6, while the height of villus, MC and the thickness of muscularis were increased significantly. With the weight gain rate as the evaluation indictor, regression analysis showed that the optimum dietary vitamin B6 requirement for sea cucumber juveniles was 45 mg/kg.