CD44、CD87和CD123在急性白血病中的表达及其与细胞免疫的相关性研究

Expression of CD44,CD87 and CD123 in Acute Leukemia and Its Correlation with Cellular Immunity

目的:探讨CD44、CD87和CD123在急性白血病(AL)中的表达及其与细胞免疫指标的相关性。方法:选择2014年5月-2017年2月以急性白血病收治的患者166例,纳入急性白血病组(包括急性髓系白血病100例,急性淋巴系白血病50例,B/髓双表型白血病16例);选择同期入院检查的非急性白血病患者50例,纳入对照组。对各组患者均取空腹静脉血5 ml,采用3色流式细胞术测定各组CD44、CD87、CD123细胞百分比;对于确诊的急性白血病患者均给予对症化疗治疗,在2个疗程后评价患者缓解率(CR),记录并统计不同疗效下CD44、CD87、CD123细胞百分比;采用SPSS Pearson相关性分析软件对急性白血病患者预后与CD44、CD87、CD123进行相关性分析。结果:急性白血病组患者CD44、CD87、CD123阳性率均高于对照组(P<0.05)。急性髓系白血病CD44、CD123阳性率均高于急性淋巴白血病和B/髓双表型(P<0.05);急性淋巴系白血病CD44阳性率高于B/髓双表型(P<0.05)。急性白血病组患者均顺利完成治疗,2个疗程后CR患者132例,PR+NR患者34例。CR患者CD44、CD87、CD123阳性率均低于PR+NR患者(P<0.05)。SPSS Pearson相关性分析结果表明:急性白血病患者预后与CD44、CD87呈负相关性(P<0.05)。结论:CD44、CD87、CD123在急性白血病不同表型中表达存在差异性,且与预后具有相关性和加强CD44、CD87、CD123测定能评估患者的预后,指导临床治疗。

Objective:To investigate the expression ot CD44,CD8/and CD123 in acute leukemia and its correlation with cellular immune markers.Methods:A total of 166 patients with acute leukemia(AL)admitted from May 2014 to February 2017 were enrolled in AL groups.Among these patients,100 patients suffered from acute myeloid leukemia,50 patients suffered from acute lymphoid leukemia,and 16 patients showed B/medullary phenotype.At the same time50 patients with non-acute leukemia were enrolled in the control group.5 ml of fasting venous blood collected from the patients in each group,and the percentage of CD44,CD 87 and CD 123 cells was determined by three-color flow cytometry.Symptomatic chemotherapy was given to the patients with confirmed acute leukemia,and the remission was evaluated after 2 treatmen courses.The Complete remission(CR)was recorded and the percentage of CD44,CD87 and CD 123 cells under different curative efficacy were recorded.The correlation of the prognosis patients with CD44,CD87 and CD123 was analyzed by SPSS Pearson correlation analysis software.Results:The positive rates of CD44,CD87 and CD 123 in AL group were all higher than those in the control group(P<0.05).The positive rates of CD44 and CD 123 in acute myeloid leukemia group were higher than those in acute lymphoblastic leukemia group and B/myeloid phenotype group(P<0.05).The positive rate of CD44 in acute lymphoid leukemia group was higher than that in B/medullary double phenotype group(P<0.05).The treatment in the patients of AL group was successfully completed.132 patients reachel to CR and 34 patients to PR+NR after 2 courses.The positive rates of CD44,CD87 and CD123 in CR patients were lower than those in PR+NR patients(P<0.05).The results of SPSS Pearson correlation analysis showed that the prognosis of patients with acute leukemia negatively correlated with CD44 and CD87(P<0.05).Conclusion:The expression of CD44,CD87 and CD 123 in different phenotype of acute leukemia are different,which correlateds with prognosis.The determination of CD44,CD87 and CD 123 can be used to evaluate the prognosis of patients for the reference of clinical treatment.