插穗因素对闽楠扦插苗生根、生长及相关酶活性的影响

Rooting,Growth and Related Enzyme Activities of Phoebe bournei Cutting Seedlings under Different Treatments of Cuttings

选择40年生无病虫害闽楠植株半木质化枝条为插穗,采用L8(27)正交试验方法,设置插穗来源(枝条顶部、枝条中部)、枝条长度(10 cm、15 cm)和留叶数(保留1片和2片全叶)3个插穗因素,研究插穗因素对闽楠扦插苗生根、生长及理化性质等方面影响,筛选适宜插穗处理方式,揭示插穗调控扦插苗生根和生长机理。结果表明:(1)闽楠插穗来源、长度及留叶数显著影响扦插苗的生根、生长及理化特性。(2)闽楠扦插最适合的插穗处理是取长度为10 cm的中部枝、保留2片叶。(3)影响闽楠扦插生根率的最主要因素是插穗来源,关键生理指标是根系PPO和POD活性;影响闽楠扦插苗新梢生长最主要的因素是插穗的留叶数,关键生理指标是根系IAAO活性和MDA含量。研究表明,各插穗因素显著影响着闽楠扦插苗的生根及生长,并以长度10 cm、保留2片的中部枝为插穗最佳;该研究结果为闽楠扦插繁殖技术制定和推广应用提供理论与技术指导。

To obtain the appropriate treatment of Phoebe bournei cutting,to explore the regulation mechanism of the rooting and growth of cutting seedlings,we used the L 8(27)orthogonal experimental design to studied effects of branch position,cutting length and leaf retention on P.bournei cutting seedling rooting,growth and physicochemical characteristics.The results showed that:(1)the branch position,cutting length and leaf retention had significant effects on the rooting,growth and physicochemical properties of P.bournei cutting seedlings.(2)For P.bournei cutting seedlings,the middle position of branch with 2 leaves and length of 10 cm as cutting was the most suitable.(3)The main factor affecting the rooting rate of P.bournei cuttings was the physiological state of cuttings,PPO and POD enzyme activities were the key physicochemical indexes.The most important factor affecting the growth of shoots of P.bournei cutting seedlings was the leaf retention,while IAAO enzyme activity and MDA content were the key physicochemical indexes.The results showed that the rooting and growth of P.bournei cutting seedlings were significantly affected by cuttings,laid a foundation for P.bournei cutting propagation and provided theoretical guidance for its application.