云南省肠炎沙门菌及德尔卑沙门菌脉冲场凝胶电泳分子分型及耐药性研究

Molecular typing by pulsed-field gel electrophoresis and drug resistance of Salmonella enteritidis and Salmonella derby in Yunnan province

目的了解云南省沙门菌患者中分离最多的肠炎沙门菌及食品中分离最多的德尔卑沙门菌脉冲场凝胶电泳(pulse field gel electrophoresis,PFGE)分子分型及耐药状况。方法参照中国细菌性传染病分子分型实验室监测网络Pulse Net China的沙门菌PFGE分子分型方法进行分子分型。分析耐药板最低抑菌浓度(minimal inhibitory concentration,MIC)值,根据美国临床实验室标准化协会(Clinical and Laboratory Standard Institute,CLSI)的相应标准获得S、I、R结果。结果56株肠炎沙门菌呈11种PFGE带型,有4种优势带型,对氨苄舒的耐药率最高,为57.14%,对亚胺培南最敏感。27株德尔卑沙门菌呈25种PFGE带型,对复合磺胺的耐药率最高,为64.29%,对亚胺培南最敏感。结论肠炎沙门菌分子分型有明显的优势带型,本地区肠炎沙门菌对氨苄舒的耐药率最高。德尔卑沙门菌分子分型呈多样分布,复方磺胺是本地区德尔脾沙门菌最耐药抗生素。2种沙门菌都对亚胺培南最敏感。

Objective To investigate pulse field gel electrophoresis molecular typing and drug resistance of themost isolated salmonella enteritidis in patients and salmonella derby in food in Yunnan province. Methods Moleculartyping of salmonella PFGE was performed by reference to Pulse Net China. Analyze the minimum inhibitoryconcentration (MIC) value of the drug-resistant plate, and obtain the S, I, R and according to the correspondingstandards of the Clinical and Laboratory Standard Institute (CLSI). Results The 56 strains of salmonella enteritidisshowed 11 PFGE bands and 4 dominant bands, with the highest resistance to ampicillin (57.14%) and the most sensitiveto imipenem (57.14%). The 27 strains of salmonella delby showed 25 PFGE types, with the highest drug resistance tosulfonamide compound (64.29%) and the most sensitive to imipenem. Conclusion The molecular classification ofSalmonella enteritidis has obvious dominant band type, and the drug resistance rate of Salmonella enteritidis toampicillin is the highest in this area. The molecular types of Salmonella derby showed diverse distribution, andcompound sulfanilamide was the most resistant antibiotic to Salmonella derby. Both Salmonella enteritidis andSalmonella derby are most sensitive to imipenem.