微小RNA-502-5p对结肠癌细胞DLD-1功能的影响

The functional effects of microRNA-502-5p on colon cancer DLD-1 cells

目的观察微小RNA(miRNA,miR)-502-5p对结肠癌细胞DLD-1功能的影响.方法选取结肠癌细胞DLD-1分别转染miR-502-5p mimic(5μl)、control mimic(5μl)、miR-502-5p inhibitor(10μl)与control inhibitor(10μl).实时定量反转录聚合酶链反应(RT-qPCR)检测miR-502-5p的表达;细胞计数试剂盒(CCK-8)实验检测细胞的增殖能力;平板克隆实验检测细胞集落形成能力;Transwell实验检测细胞迁移与侵袭能力.结果转染miR-502-5p mimic与NC mimic后,DLD-1细胞中miR-502-5p的相对表达量分别为319.31±33.16和1.08±0.22,DLD-1细胞中miR-502-5p提高显著(P<0.05);转染miR-502-5p inhibitor与NC inhibitor后DLD-1细胞中miR-502-5p的相对表达量分别为0.28±0.04和0.99±0.11,DLD-1细胞中miR-502-5p显著降低(P<0.05);转染miR-502-5p mimic后DLD-1细胞的增殖能力:miR-502-5p mimic实验组[(125±9)个]与controlmimic组[(112±14)个]之间差异无统计学意义(P>0.05),而迁移[(621±90)个比(174±32)个]与侵袭[(531±45)个比(132±34)个]能力显著提高(P<0.05);转染miR-502-5p inhibitor后DLD-1细胞的增殖能力:miR-502-5p inhibitor实验组[(118±22)个]与control inhibitor组[(100±15)个]之间差异无统计学意义(P>0.05),而迁移[(133±50)个比(543±84)个]与侵袭[(119±45)个比(458±57)个]能力显著降低(P<0.05).结论miR-502-5p可促进DLD-1细胞的迁移与侵袭能力,但是不影响增殖功能.

Objective To investigate the effect of microRNA(miRNA,miR)-502-5p on colon cancer DLD-1 cells.Methods Colon cancer cell line DLD-1 was transfected by miR-502-5p mimic,control mimic,miR-502-5p inhibitor and control inhibitor respectively.The effect of miR-502-5p on the proliferation of DLD-1 cells was detected by cell counting kit-8(CCK-8)assay.The effect of miR-502-5p on the colony forming ability of DLD-1 cells was detected by plate-cloning assay.Transwell assay was used to evaluate the effect of miR-502-5p on the migration and invasion of colon cells.Results The expression of miR-502-5p was significantly increased(319.31±33.16 vs.1.08±0.22)in DLD-1 cells after transfection with miR-502-5p mimic(P<0.05).miR-502-5p was significantly decreased(0.28±0.04 vs.0.99±0.11)in DLD-1 cells after transfection with miR-502-5p inhibitor(P<0.05).After transfection of miR-502-5p mimic,the proliferation and colony forming ability of DLD-1 cells[(125±9)cells vs.(112±14)cells]were not significantly changed(P>0.05),while migration[(621±90)cells vs.(174±32)cells]and invasion[(531±45)cells vs.(132±34)cells]ability were significantly increased(P V 0.05).The proliferation and colony forming ability of DLD-1 cells[(118±22)cells vs.(100±15)cells]were not significantly changed after transfection of miR-502-5p inhibitor(P>0.05),but the migration[(133±50)cells vs.(543±84)cells]and invasion[(119±45)cells vs.(458±57)cells]ability were decreased significantly(P<0.05).Conclusion MiR-502-5p may promote the migration and invasion of DLD-1 cells,but has no effect on the proliferation function.