基于SLAF-seq技术的甘薯种质资源群体遗传进化分析

Phylogenetic Analysis of Sweetpotato Germplasm Resources Based on SLAF-seq Technology

基于简化基因组测序技术对122份甘薯种质资源进行分析,开发SNP位点并将其应用于种质资源群体结构和遗传进化分析。结果表明,从122份甘薯种质资源中共获得563.18 Mb读长,不同材料的读长数量在1 419 809~8 392 785范围之间。测序质量值Q30在90.61%~96.82%之间,平均Q30为92.78%。测序获得的GC含量在36.46%~40.33%之间,平均GC含量为38.17%,对照GC含量为40.72%。根据测序结果共开发出高质量的SLAF标签2388759个,平均测序深度为17.45’。其中,多态性的SLAF标签77761个,占SLAF标签总数的3.26%。根据所获得多态性SLAF标签统计SNP位点信息,共计获得129 063个群体SNP位点。遗传进化分析表明122份种质资源可以分为3个大类,分类结果与它们的地理来源无直接关系,聚类结果可为甘薯育种亲本组配和杂种优势利用提供科学依据。

Based on SLAF-seq technology, 122 sweet potato germplasm resources were used to develop SNP sites, which were applied to the analysis of population structure and genetic evolution on all germplasm resources. Results showed that 563.18 Mb reads length was obtained by sequencing, the reads of the samples varied from 1 419 809 to 8 392 785. The sequencing quality value(Q30) changed from 90.61% to 96.82%, and the average Q30 was 92.78%. The GC content of the samples changed from 36.46%?40.33%, the average value was 38.17%, and that of the control was 40.72%. A total of 2 388 759 SLAF tags were developed, with an average sequencing depth of 17.45?. There were 77 761 polymorphic SLAF tags accounting for 3.26% of the total SLAF tags. Finally, 129 063 SNPs were found. The population structure and genetic evolution analysis showed that 122 germplasm resources could be divided into three groups, which was not directly related to the geographical source, and the results of clustering could provide scientific basis for parents combination and utilization of heterosis in sweetpotato breeding.