苏叶黄连汤抗流感活性成分提取工艺的优化及目标相关性分析

Extraction process optimization for active anti-influenza components from Suye Huanglian Decoction and correlation analysis among objectives

目的优化苏叶黄连汤抗流感活性成分提取工艺,并进行目标相关性分析。方法以液料比、提取温度、提取时间、提取次数为影响因素,迷迭香酸含有量、盐酸小檗碱含有量、浸膏得率、流感病毒抑制率为评价指标,均匀设计多目标遗传算法优化提取工艺。然后,Pearson分析考察流感病毒抑制率与各目标之间的相关性。结果最佳条件为液料比28∶1,提取温度97℃,提取时间108 min,提取次数3次,迷迭香酸含有量、盐酸小檗碱含有量、浸膏得率、流感病毒抑制率分别为241mg/g、3092mg/g、283%、668%。流感病毒抑制率与2种成分含有量呈显著正相关(P<005)。结论该方法稳定可靠,可用于提取苏叶黄连汤抗流感活性成分。

AIM To optimize the extraction process for active anti influenza components from Suye Huanglian Decoction,and to perform correlation analysis among objectives.METHODS With liquid solid ratio,extraction temperature,extraction time and extraction frequency as influencing factors,rosmarinic acid content,berberine hydrochloride content,extract yield and inhibitory rate on influenza virus as evaluation indices,the extraction process was optimized by uniform design(multi objective)genetic algorithm.Subsequently,Pearson analysis was applied to investigating the correlations between inhibitory rate on influenza virus and various objectives.RESULTS The optimal conditions were determined to be 28∶1 for liquid solid ratio,97℃for extraction tem perature,108 min for extraction time,and three times for extraction frequency,the rosmarinic acid content,ber berine hydrochloride content,extract yield and inhibitory rate on influenza virus were identified to be 241 mg/g,3092 mg/g,283%and 668%,respectively.The inhibitory rate on influenza virus demonstrated significant posi tive correlations with the contents of two constituents(P<005).CONCLUSION This stable and reliable method can be used for the extration of active anti influenza components from Suye Huanglian Decoction.