肚液散GC指纹图谱建立及60Co-γ辐照灭菌对4种成分的影响

Establishment of GC fingerprints of Duye Powder and effects of 60Co-γ irradiation sterilization on four constituents

目的建立肚液散(甘草、麦芽、鸡内金等)GC指纹图谱,并考察60Coγ辐照灭菌对乙酸龙脑酯、桂皮醛、丁香酚、肉豆蔻醚含有量的影响。方法该药物乙酸乙酯提取液的分析采用HP 5毛细管柱(032mm×30m,025μm);进样口温度240℃;FID检测器温度260℃。结果13批样品指纹图谱中有13个共有峰,相似度大于0930。4种成分在各自范围内线性关系良好(r≥09997),平均加样回收率9362%~1071%,RSD23%~32%。辐照灭菌后,桂皮醛、丁香酚、肉豆蔻醚含有量无明显变化(P>005);乙酸龙脑酯含有量在40kGy下较稳定,在80kGy下显著降低(P<005)。结论60Co-γ辐照灭菌对肚液散挥发油成分稳定性有一定影响,故在保证灭菌效果前提下应选择低辐照剂量。

AIM To establish the GC fingerprints of Duye Power(Glycyrrhizae Radix et Rhizoma,Hordei Fructus Germinatus,Galli gigerii Endothelium Corneum,etc.)and to investigate the effects of 60Co⁃γirradiation sterilization on bornyl acetate,cinnamaldehyde,eugenol and myristicin contents.METHODS The analysis of ethyl acetate extract of this drug was performed on an HP⁃5 capillary column(30 m×0.32 mm,0.25 m),and the injector temperature and detector temperature were 240℃and 260℃,respectively.RESULTS There were thir⁃teen common peaks in the fingerprints of thirteen batches of samples with the similarties of more than 0􀆰930.Four constituents showed good linear relationships within their own ranges(r≥0.9997),whose average recoveries were 93􀆰62%-107.1%with the RSDs of 2.3%-3.2%.After irradiation sterilization,cinnamaldehyde,eugenol and myristicin contents demonstrated no obvious changes(P>0.05);bornyl acetate content was stable at 4.0 kGy,which was significantly decreased at 8.0 kGy(P<0􀆰05).CONCLUSION 60 Co⁃γirradiation sterilization has a certain influence on the stability of volatile components in Duye Powder,so low irradiation dose is suggested for use on the premise of ensuring sterilization effect.