栀子标准汤剂的量值传递规律

Quantity transferring rules of Gardeniae Fructus standard decoction

目的探讨栀子标准汤剂的量值传递规律。方法以栀子苷含有量为评价指标,70%甲醇代替甲醇(2015年版《中国药典》规定)作为提取溶剂,超声时间由20 min延长至40 min。建立标准汤剂UPLC特征图谱后,根据出膏率、指标成分转移率、特征峰传递数考察量值传递规律。结果新方法所测得栀子苷含有量较药典法高出近30%。15批样品特征图谱中有6个特征峰,平均出膏率为27.7%,栀子苷平均转移率为82.4%,特征峰传递数为6。结论栀子苷含有量测定方法改进后,出膏率、指标成分转移率、特征峰传递数可反映栀子标准汤剂量值传递规律。

AIM To explore the quantity transferring rules of Gardeniae Fructus standard decoction.METHODS Taking geniposide content as an evaluation index,70%methanol was taken as an extraction solvent instead of methanol[described in the Chinese Pharmacopoeia(2015 edition)],and ultrasonic time was extended from 20 min to 40 min.The UPLC characteristic chromatogram of standard decoction was established,after which quantity transferring rules were investigated according to paste forming rate,transferring rate of index components and transferring number of characteristic peaks.RESULTS The geniposide content determined by new method was about 30%higher than that determined by pharmacopoeia method.There were six characteristic peaks in the characteristic chromatograms of fifteen batches of samples,the average paste forming rate,transferring rate of index components and transferring number of characteristic peaks were 27.7%,82.4%and 6,respectively.CONCLUSION After the content determination method of geniposide is improved,paste forming rate,transferring rate of index components and transferring number of characteristic peaks can reflect the quantity trans ferring rules of Gardeniae Fructus standard decoction.