Genome-wide identification and prediction of long noncoding RNAs in half-smooth tongue sole Cynoglossus semilaevis

At present,the function and profile of long noncoding RNAs(lncRNAs)in half-smooth tongue soles Cynoglossus semilaevis remain poorly understood.Therefore,we identified lncRNAs in the species using large-scale deep sequencing approaches.A total of 96726222 clean reads and 2497 lncRNAs were obtained from the compound samples.In total,1694 known lncRNAs and 803 novel lncRNAs were identified.Most of the novel lncRNAs distributed mainly in a range of 200-3000 nt and contained 2-6 exons.Six novel lncRNAs were selected for expression analysis by quantitative reverse transcription-polymerase chain reaction,of which lnc_770 and lnc_150 were expressed mainly in the ovary.This study provides a valuable resource for lncRNA studies of half-smooth tongue sole and improves our understanding of the function of non-coding RNA in fish.

At present, the function and pro?le of long noncoding RNAs（lncRNAs） in half-smooth tongue soles Cynoglossus semilaevis remain poorly understood. Therefore, we identi?ed lncRNAs in the species using large-scale deep sequencing approaches. A total of 96 726 222 clean reads and 2497 lncRNAs were obtained from the compound samples. In total, 1 694 known lncRNAs and 803 novel lncRNAs were identi?ed. Most of the novel lncRNAs distributed mainly in a range of 200–3 000 nt and contained 2–6 exons. Six novel lncRNAs were selected for expression analysis by quantitative reverse transcription-polymerase chain reaction, of which lnc770 and lnc150 were expressed mainly in the ovary. This study provides a valuable resource for lncRNA studies of half-smooth tongue sole and improves our understanding of the function of non-coding RNA in ?sh.