Cullin7蛋白在人脑胶质瘤中的表达及临床意义

Expression and significance of Cullin7 protein in human gliomas

目的探讨Cullin7蛋白在人脑胶质瘤中的表达及临床意义。方法选取2013年1月至2018年12月手术切除脑胶质瘤标本60例,其中WHO分级Ⅰ级12例,Ⅱ级18例,Ⅲ级14例,Ⅳ级16例。另选取重型颅脑损伤内减压术切除正常脑组织10例作为对照组。采用免疫组化染色、免疫印迹法法检测Cullin7蛋白表达情况。51例随访46个月,9例失访;采用Kaplan-Meir法分析和Log-rank检验分析生存曲线。结果高级别胶质瘤Cullin7蛋白阳性表达率(66.7%)明显高于低级别胶质瘤(36.7%;P<0.05),而低级别胶质瘤Cullin7蛋白阳性表达率明显高于对照组(0%;P<0.05)。Cullin7高表达病人生存期较低表达病人明显缩短(P<0.05)。结论Cullin7蛋白在脑胶质瘤中的表达与肿瘤恶性程度呈正相关,与病人生存期呈负相关。

Objective To investigate the expression and significance of Cullin7 in human brain gliomas.Methods The expression of Cullin7 protein was determined by immunohistochemical technique and Western blot in 60 samples of gliomas,of which,12 belonged in WHO gradeⅠ,18 gradeⅡ,14 gradeⅢand 16 gradeⅣgained from the patients with gliomas undergoing neurosurgery in our hospital from 2013 to 2018,and 10 normal cerebral tissues gained from patients with severe traumatic brain injury undergoing internal decompression.The relationship of the survival time with the expression of Cullin7 in the gliomas tissues was analyzed in the patients with different WHO grades of gliomas.Results The level of Cullin7 protein expression was significantly higher in the gliomas tissues than that in normal brain tissues(P<0.05),and the level of Cullin7 protein expression was significantly higher in the high-grade gliomas(WHO gradesⅢ~Ⅳ)tissues than that in the low-grade gliomas(WHO gradesⅠ~Ⅱ)tissues(P<0.05).The Survival time of patients with high expression of Cullin7 was significantly shorter than those with low expression of Cullin7(P<0.05).Conclusion The expression of Cullin7 protein in the gliomas tissues was positively correlated with tumor malignancy and negatively correlated with the survival time in the patients with brain gliomas.